Anti-BMP2 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P12643

Core Product Specifications and Parameters

Biological Background: BMP2 Function and Localization

• BMP2 (Bone morphogenetic protein 2), also known as BMP-2A, is a member of the TGF-beta superfamily that plays essential roles in developmental processes, including cardiogenesis, neurogenesis, and osteogenesis. Related references: PMID:18436533, PMID:24362451, PMID:31019025
• It induces cartilage and bone formation by initiating the canonical BMP signaling cascade through association with type I receptor BMPR1A and type II receptor BMPR2. Related references: PMID:3201241, PMID:15064755, PMID:17295905
• Upon receptor binding, BMPR2 phosphorylates and activates BMPR1A, which then phosphorylates SMAD1/5/8 to regulate transcription of target genes. Related references: PMID:7791754
• BMP2 can also signal through non-canonical pathways such as ERK/MAP kinase signaling to regulate osteoblast differentiation. Related references: PMID:16771708, PMID:20851880
• It stimulates myoblast differentiation into osteoblasts via the EIF2AK3-EIF2A-ATF4 pathway, increasing ATF4 expression. Related references: PMID:24362451
• The protein is secreted and exhibits widespread tissue expression, with particular abundance in lung, spleen, and colon; it is also present in heart, brain, placenta, liver, kidney, and pancreas.
• BMP2 acts as a positive regulator of odontoblast differentiation during tooth germ formation and promotes HAMP expression through BMPR1A/ALK3. Related references: PMID:31800957

Experimental Guidance and Technical Tips

• The immunogen corresponds to a recombinant fusion protein containing amino acids 283-396 of human BMP2; consider potential cross-reactivity with other BMP family members sharing homologous regions.
• In Western blot, the full-length BMP2 appears as a ~45kDa band, while the mature active form is approximately 18kDa; validate detection in your specific samples.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), antigen retrieval and optimization of antibody dilution and detection conditions may be necessary due to the secreted nature of BMP2.
• ELISA applications can utilize this antibody as capture or detection; confirm optimal working concentrations and compatibility with your assay format.
• When working with mouse or rat samples, validate reactivity given high sequence similarity; positive controls are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive one-stop platform for IVD raw material sourcing, technical support, and consulting. This BMP2 antibody supports research in osteogenesis, developmental biology, and related signaling pathways. Beyond individual antibodies, we offer validated antibody pairs, bulk ancillary reagents, and access to rare targets to accelerate your assay development from concept to clinic.