Anti-BMP2 Rabbit Monoclonal Antibody for WB, IF/ICC, IP, ELISA - P12643

Core Product Specifications and Parameters

Biological Background: BMP2 Function and Localization

• BMP2 (Bone morphogenetic protein 2A) is a secreted glycoprotein and TGF-beta superfamily growth factor crucial for cardiogenesis, neurogenesis, and osteogenesis. Related references: PMID:18436533 PMID:24362451 PMID:31019025

• It induces cartilage and bone formation. Related references: PMID:3201241

• Canonical signaling involves binding to BMPR1A/BMPR2 receptors, leading to SMAD1/5/8 phosphorylation and nuclear translocation to regulate gene transcription. Related references: PMID:15064755 PMID:17295905 PMID:18436533 PMID:7791754

• It promotes hepcidin (HAMP) expression via BMPR1A/ALK3 receptor interaction. Related references: PMID:31800957

• Non-canonical signaling includes ERK/MAP kinase activation regulating osteoblast differentiation. Related references: PMID:16771708 PMID:20851880

• BMP2 stimulates myoblast-to-osteoblast differentiation through the EIF2AK3-EIF2A-ATF4 pathway. Related references: PMID:24362451

• It positively regulates odontoblast differentiation during tooth development.

• BMP2 is particularly abundant in lung, spleen, and colon, with lower expression in heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, ovary, and small intestine.

Experimental Guidance and Technical Tips

• The recombinant protein immunogen suggests the antibody can detect both denatured and native BMP2. For WB, use a positive control lysate from lung or spleen, and expect a band around 45 kDa.
• For IF/ICC, optimize fixation and permeabilization protocols as BMP2 is secreted; co-staining with Golgi markers may improve signal.
• For IP, titrate antibody concentration and use appropriate lysis buffers to maintain protein interactions.
• For ELISA, validate using recombinant BMP2 as a standard to ensure assay compatibility.
• The antibody cross-reacts with mouse and rat BMP2, making it suitable for orthologous studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop B2B sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. This anti-BMP2 antibody supports research in developmental biology and bone morphogenesis. Contact us to streamline your procurement from concept to clinic.