Anti-BMP10 Polyclonal Antibody for WB, ELISA - O95393

Core Product Specifications and Parameters

Biological Background: BMP10 Function and Localization

• BMP10 is a secreted bone morphogenetic protein and member of the TGF-beta superfamily, functioning as a ligand for type I receptors ACVRL1/ALK1, BMPR1A/ALK3, and BMPR1B/ALK6.
• Upon receptor binding, it activates the SMAD1/5/8 signaling pathway, which plays critical roles in developmental processes.
• In embryonic development, BMP10 is essential for maintaining cardiomyocyte proliferation by preventing premature activation of CDKN1C/p57KIP and sustaining expression of cardiogenic factors like MEF2C and NKX2-5.
• It inhibits endothelial cell migration and growth, suggesting a role in vascular biology and angiogenesis regulation.
• In breast cancer cell lines, BMP10 may reduce cell migration and cell-matrix adhesion, indicating potential tumor-suppressive functions.
• The protein is detected in mammary epithelia at the protein level, hinting at tissue-specific roles beyond the cardiovascular system.
• BMP10 is a disulfide-linked homodimer, undergoes cleavage at a pair of basic residues during maturation, and is N-glycosylated prior to secretion.
• Keywords associated with BMP10 include developmental protein, cytokine, growth factor, and disease variant, reflecting its involvement in congenital heart defects and other conditions.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 22-316 of human BMP10, covering nearly the entire mature protein; thus, the antibody is expected to recognize full-length BMP10 and potentially its precursor form, depending on sample preparation.
• For Western blot (WB), a band around 48kDa corresponds to the monomeric form under reducing conditions, though dimer formation may be observed under non-reducing conditions.
• Cross-reactivity with rat BMP10 is reported; validate in the relevant sample system using cardiac or mammary tissue lysates.
• ELISA applications may require optimizing coating conditions due to the secreted nature of BMP10; consider using capture/detection antibody pairs for sandwich ELISA configurations.
• Since BMP10 is secreted, cell culture supernatants or tissue extracts with appropriate secretion-blocking agents (e.g., Brefeldin A) may be necessary to detect intracellular or secreted pools.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents tailored to diagnostic developers. This BMP10 polyclonal antibody supports research into cardiac development and cancer, and CamelBio can assist in scaling procurement for immunoassay development. With expertise in rare target sourcing and supply chain management, CamelBio bridges the gap from concept to clinic.