Anti-Bmi1 Rabbit Monoclonal Antibody for WB, ELISA - P35226

Core Product Specifications and Parameters

Biological Background: Bmi1 Function and Localization

• BMI-1 is the core component of the PRC1-like Polycomb group complex, which maintains the transcriptionally repressive state of many genes, including Hox genes, throughout development. Related references: PMID:15386022, PMID:16359901, PMID:16714294
• The BMI-1‐containing complex mediates monoubiquitination of histone H2A at Lys-119, rendering chromatin heritably changed in its expressibility. Related references: PMID:21772249, PMID:25355358
• BMI-1 regulates the E3 ubiquitin-protein ligase activity of RNF2/RING2 within the PRC1-like complex. Related references: PMID:15386022, PMID:21772249, PMID:26151332
• Subcellular localization: BMI-1 is found in both the nucleus and the cytoplasm.
• Functional keywords include chromatin regulator, proto-oncogene, transcription repressor, zinc-finger protein, and metal-binding.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 227-326 of human Bmi1; for WB specificity validation, consider using a blocking peptide or antigen competition assay.
• Given the confirmed cross-reactivity with rat, this antibody may be suitable for rat model studies; perform pilot experiments to confirm performance in your sample system.
• For ELISA applications, optimize the coating antigen concentration and detection conditions to match the expected Bmi1 abundance in your analyte.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to validated IVD raw materials, including a comprehensive portfolio of monoclonal antibodies for chromatin biology targets like Bmi1. Whether you require bulk quantities of this anti-Bmi1 antibody, complementary ancillary reagents, or custom sourcing for rare epigenetic targets, CamelBio’s one-stop platform bridges the gap from concept to clinic. Contact our team to learn how we can support your diagnostic development with reliable and efficient supply solutions.