Anti-Bmi1 Polyclonal Antibody for WB, IF/ICC, ELISA - P35226

Core Product Specifications and Parameters

Biological Background: Bmi1 Function and Localization

• Polycomb complex protein BMI-1 (also known as PCGF4, RNF51) is a core component of the Polycomb repressive complex 1 (PRC1), which maintains transcriptional repression of key developmental genes, including Hox clusters, through chromatin remodeling and histone modifications.
• Within the PRC1 complex, BMI-1 collaborates with RNF2/RING2 to catalyze the monoubiquitination of histone H2A at Lys-119 (H2AK119ub), a critical epigenetic mark for stable gene silencing. Related references: PMID:15386022, PMID:16359901, PMID:16714294
• The RNF2-UB2D3-BMI1 ternary complex specifically binds to nucleosomes and exerts its E3 ubiquitin ligase activity solely on nucleosomal histone H2A, highlighting its chromatin-targeted function. Related references: PMID:21772249, PMID:25355358
• BMI-1 acts as a positive regulator of RNF2's ubiquitin ligase activity, thereby enhancing PRC1-mediated H2A ubiquitination and reinforcing polycomb-mediated transcriptional repression. Related references: PMID:21772249, PMID:26151332
• Subcellular localization is predominantly nuclear, consistent with its role in chromatin regulation, though cytoplasmic distribution has also been observed under certain conditions (UniProt: SUBCELLULAR LOCATION).
• BMI-1 is classified as a proto-oncogene and chromatin regulator, featuring a RING finger domain that coordinates zinc ions essential for its structural integrity and function (UniProt: KEYWORDS).

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 120-326 of human BMI-1, a central region that may yield antibodies recognizing multiple conformations or interaction states; validate specificity in the relevant sample system.
• For Western blotting, the predicted molecular weight is approximately 37 kDa; consider using a loading control of similar size and optimize lysis conditions to preserve protein integrity.
• In immunofluorescence/immunocytochemistry, fixation and permeabilization protocols can impact antigen accessibility; testing different methods (e.g., methanol vs. formaldehyde) may improve nuclear staining.
• ELISA performance can be verified using the recombinant immunogen as a positive control; include appropriate negative controls to assess background binding.
• As a polyclonal antibody, lot-to-lot consistency should be monitored when transitioning between batches, and re-optimization of dilution factors may be necessary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined sourcing platform for IVD raw materials, providing diagnostic manufacturers and research institutions with access to validated products and flexible supply solutions. This anti-BMI1 antibody supports investigations into epigenetic regulation, stem cell biology, and cancer research, complementing our portfolio of chromatin-related reagents. Beyond individual antibodies, we supply matched antibody pairs, optimized monoclonal/polyclonal preparations, bulk ancillary reagents, and can facilitate custom sourcing for rare or emerging targets, helping you accelerate your projects from concept to clinic.