Anti-BLVRB Rabbit Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P30043

Core Product Specifications and Parameters

Biological Background: BLVRB Function and Localization

• BLVRB is a dual-function enzyme that acts as an NAD(P)H-dependent reductase and an S-nitroso-CoA-dependent nitrosyltransferase, playing critical roles in cellular metabolism and signaling. Related references: PMID:10620517 PMID:18241201 PMID:27207795
• During fetal development, BLVRB promotes heme catabolism by catalyzing the reduction of biliverdin IXbeta to bilirubin IXbeta, a major fetal heme degradation product distinct from the adult pathway. Related references: PMID:10858451 PMID:18241201 PMID:7929092
• In adult tissues, BLVRB regulates hematopoiesis and intermediary metabolism, influencing glutaminolysis, glycolysis, the TCA cycle, and the pentose phosphate pathway, as well as modulating insulin signaling. Related references: PMID:27207795 PMID:29500232 PMID:38056462
• The enzyme exhibits broad oxidoreductase activity, reducing a variety of substrates including flavins (riboflavin, FAD, FMN), biliverdins, methemoglobin, and PQQ. Related references: PMID:10620517 PMID:18241201 PMID:7929092
• BLVRB functions as a protein nitrosyltransferase, mediating S-nitrosylation of cysteine residues on target proteins such as HMOX2, INSR, and IRS1, thereby inhibiting insulin receptor signaling. Related references: PMID:38056462
• Localized predominantly in the cytoplasm, BLVRB is highly expressed in liver and erythrocytes, with lower levels detected in heart, lung, adrenal gland, and cerebrum; it is also present in adult red blood cells. Related references: PMID:7929092 PMID:29932944
• BLVRB is subject to phosphorylation and has been structurally characterized; it is annotated as a reference proteome component with both oxidoreductase and transferase activities.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human BLVRB protein (amino acids 1–206), which may provide broad recognition of the target in native and denatured conditions. Consider validating specificity in knockout or knockdown models for critical applications.
• For Western blot (WB), a specific band around 22 kDa is expected; use appropriate loading controls and consider reducing conditions. Cross-reactivity with mouse and rat BLVRB allows multi-species studies, but validate in the relevant sample system.
• In immunofluorescence (IF/ICC), BLVRB exhibits cytoplasmic staining; optimize fixation and permeabilization protocols to preserve epitope accessibility and cellular morphology.
• For immunohistochemistry (IHC-P), antigen retrieval methods (heat-induced or enzymatic) may enhance detection in formalin-fixed paraffin-embedded tissues; include appropriate controls to confirm specificity.
• This polyclonal antibody is suitable for ELISA applications; determine optimal coating concentrations and detection conditions empirically for your assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw-material sourcing, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target procurement to streamline diagnostic development. For researchers and manufacturers focused on heme metabolism, redox biology, or insulin signaling, CamelBio provides comprehensive access to core reagents like this BLVRB antibody, along with technical consultation to support your workflow from concept to clinic. Our supply capabilities ensure you receive consistent, high-quality materials tailored to your assay needs—reducing lead times and enabling reliable performance.