Anti-BLOC1S3 Rabbit Polyclonal Antibody for IHC-P, IF/ICC, ELISA - Q6QNY0

Core Product Specifications and Parameters

Biological Background: BLOC1S3 Function and Localization

• Protein identity and aliases: Biogenesis of lysosome-related organelles complex 1 subunit 3 (BLOC1S3), also known as RP, HPS8, and BLOS3.
• Core function: BLOC1S3 is a component of the BLOC-1 complex, essential for the biogenesis of lysosome-related organelles (LROs) including platelet dense granules and melanosomes.
• Cargo trafficking role: Together with the AP-3 complex, BLOC-1 targets membrane protein cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals.
• Neurite extension: In association with SNARE proteins, the BLOC-1 complex is proposed to contribute to neurite extension.
• Vesicle trafficking: Plays a role in intracellular vesicle trafficking.
• Subcellular localization: Predominantly localized in the cytoplasm.
• Disease association: Mutations in BLOC1S3 are associated with Hermansky-Pudlak syndrome type 8 (HPS8), characterized by albinism and platelet storage pool deficiency.
• Post-translational modification: Identified as a phosphoprotein.

Experimental Guidance and Technical Tips

• As a polyclonal antibody generated against full-length recombinant BLOC1S3, it may recognize multiple epitopes, potentially providing strong signal in applications such as IHC-P, IF/ICC, and ELISA.
• For IHC-P, consider optimizing antigen retrieval conditions and primary antibody dilution using appropriate positive (e.g., human skin or platelet) and negative controls.
• For IF/ICC, fixation and permeabilization should be validated in the relevant cell system; note that BLOC1S3 is cytoplasmic.
• In ELISA, the recombinant immunogen can be used as a positive control to assess antibody titer.
• Cross-reactivity with mouse and rat is reported; validate species-specific reactivity in your assay context if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. This anti-BLOC1S3 antibody supports research into lysosome-related organelle biology, platelet disorders, and Hermansky-Pudlak syndrome. Beyond antibodies, CamelBio supplies validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing, streamlining your development from concept to clinic.