Anti-BLOC1S2 Rabbit Polyclonal Antibody for WB, ELISA - Q6QNY1

Core Product Specifications and Parameters

Biological Background: BLOC1S2 Function and Localization

• BLOC1S2, also known as centrosome-associated protein (CEAP), BLOS2, or BORCS2, is a component of the biogenesis of lysosome-related organelles complex 1 (BLOC-1) that is essential for normal biogenesis of lysosome-related organelles (LROs) such as platelet dense granules and melanosomes. Related references: PMID:15102850 PMID:17182842
• In neurons, BLOC-1 cooperates with the AP-3 complex to target membrane protein cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals, and is also proposed to be involved in neurite extension.
• BLOC1S2 is also a subunit of the BORC complex, which associates with the cytosolic face of lysosomes and recruits ARL8B to couple lysosomes to microtubule plus-end-directed kinesin motor, thereby regulating lysosome movement and peripheral localization. Related references: PMID:25898167
• It may play a role in cell proliferation. Related references: PMID:15381421
• Subcellularly, BLOC1S2 localizes to the cytoplasm, centrosome (microtubule organizing center), and lysosome membrane.
• Alternative splicing generates multiple isoforms; isoform 1 and isoform 2 are widely expressed, including in various malignant tumor tissues at the protein level.
• Post-translational modifications include acetylation, and the protein features a coiled-coil domain.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 44–142 of human BLOC1S2 (NP_776170.2), a region sharing high sequence identity with mouse and rat orthologs, supporting the reported cross-reactivity. For western blot, consider testing a dilution range of 1:500–1:2000 using whole‑cell lysates from human, mouse, or rat samples; verify the target band at approximately 16 kDa.
• For ELISA, coat the plate with recombinant BLOC1S2 protein or peptide and use this polyclonal antibody for detection. Optimize coating and antibody concentrations for your specific assay format.
• As a polyclonal antibody, lot‑to‑lot consistency should be assessed when transitioning to new batches, especially for quantitative or regulated applications. Incorporate appropriate positive and negative controls in all experiments.
• While the antibody is validated for WB and ELISA, it may also be suitable for other applications such as immunoprecipitation or immunohistochemistry; however, independent validation in the relevant sample system is essential.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic and life science teams with a one‑stop portal for IVD raw materials. This BLOC1S2 rabbit polyclonal antibody complements our portfolio for lysosome‑related organelle and intracellular trafficking research. Beyond individual antibodies, we offer validated antibody pairs, bulk ancillary reagents, and customized sourcing solutions to streamline your assay development from concept to clinic.