Anti-BLBP/FABP7 Rabbit Monoclonal Antibody for WB, IF-P, ELISA - O15540

Core Product Specifications and Parameters

Biological Background: BLBP/FABP7 Function and Localization

• Encoded by the FABP7 gene, fatty acid-binding protein, brain (BLBP/FABP7) is also known as brain-type fatty acid-binding protein (B-FABP), mammary-derived growth inhibitor related (MRG), and BLBP/FABP7.
• B-FABP could be involved in the transport of a so far unknown hydrophobic ligand with potential morphogenic activity during central nervous system (CNS) development.
• It is required for the establishment of the radial glial fiber system in developing brain, a system necessary for the migration of immature neurons to establish cortical layers (By similarity).
• Located in the cytoplasm, it functions as a lipid chaperone that may influence signaling and metabolic pathways in neural tissues.
• Tissue specificity is primarily in brain and other neural tissues, consistent with its role in neurodevelopment.
• Belongs to the fatty acid-binding protein family; the protein exhibits lipid-binding and transport activities.
• Post-translational modifications include acetylation and alternative splicing, with a canonical molecular weight of ~14.9 kDa and additional isoforms (e.g., 18 kDa) reported.
• Its three-dimensional structure has been solved, and direct protein sequencing corroborates its identity (UniProt keywords: 3D-structure, Acetylation, Alternative splicing, Direct protein sequencing, Lipid-binding, Transport).

Experimental Guidance and Technical Tips

• As a rabbit monoclonal antibody raised against a synthetic peptide spanning the N-terminal 100 amino acids of human BLBP/FABP7, epitope specificity is confined to that region; confirm recognition of both 14 kDa and 18 kDa isoforms in your system.
• For Western blot (WB) validation, consider using a loading control with a similar molecular weight range (10–20 kDa) and verify detection in human or mouse brain lysates.
• For immunofluorescence on paraffin-embedded sections (IF-P), validate staining patterns in brain tissue sections, where BLBP/FABP7 is predominantly cytoplasmic; include appropriate negative controls.
• In ELISA applications, optimize coating conditions and antibody dilution; cross-reactivity with mouse ortholog suggests utility in comparative studies.
• Given the protein’s role in radial glial fiber development, in vitro models of neurogenesis or gliogenesis may serve as functionally relevant positive controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines procurement for diagnostic and research laboratories by providing high-quality IVD raw materials like the BLBP/FABP7 rabbit monoclonal antibody, catering to neuroscience and lipid-binding research. Beyond this target, we offer comprehensive sourcing support through validated antibody pairs, optimized bulk ancillary reagents, and access to rare-target antibodies, helping you bridge discovery to clinical application with efficiency.