Anti-BIRC7 (KIAP/Livin/ML-IAP) Rabbit Polyclonal Antibody - Q96CA5

Core Product Specifications and Parameters

Biological Background: BIRC7 Function and Localization

• BIRC7, also known as Livin, KIAP, ML-IAP, or RNF50, is a member of the inhibitor of apoptosis (IAP) family that contains a baculoviral IAP repeat (BIR) domain and a RING finger domain.
• BIRC7 is a dual-function apoptotic regulator, exerting both pro- and anti-apoptotic activities; its anti-apoptotic function involves inhibition of CASP3, CASP7, and CASP9, and E3 ubiquitin-protein ligase-mediated degradation of SMAC/DIABLO to relieve XIAP inhibition. Related references: PMID:11024045, PMID:16729033
• It protects cells from apoptosis induced by TNF, adriamycin, etoposide, or staurosporine, with suppression mediated by MAPK8/JNK1 and MAPK9/JNK2 activation via TAB1 and MAP3K7/TAK1. Related references: PMID:11084335, PMID:11162435, PMID:11865055
• The protein additionally blocks staurosporine- and etoposide-induced apoptosis and modulates natural killer cell-mediated cytotoxicity. Related references: PMID:11322947, PMID:11162435, PMID:18034418
• It localizes to the nucleus, cytoplasm, and Golgi apparatus.
• Isoforms 1 and 2 show low or undetectable expression in most adult tissues but are detected in placenta, lung, lymph node, spleen, ovary, and various carcinoma cell lines; isoform 2 is additionally found in fetal kidney, heart, and spleen.
• BIRC7 is classified as a protease inhibitor and thiol protease inhibitor; it contains zinc-finger motifs and participates in ubiquitin conjugation pathways.

Experimental Guidance and Technical Tips

• The immunogen (aa 1-265) encompasses the BIR domain, likely generating antibodies that recognize both BIRC7 isoforms; verify isoform specificity if required for your experimental design.
• For Western blotting, expect a band at ~33 kDa; consider using a low-percentage gel to resolve close molecular weight species and validate with appropriate controls (e.g., siRNA knockdown or overexpression lysates).
• This polyclonal antibody is qualified for WB, IHC-P, IF/ICC, and ELISA. For immunohistochemistry, optimize antigen retrieval and antibody dilution using positive control tissues such as placenta or carcinoma samples known to express BIRC7.
• Cross-reactivity with mouse and rat enables use in rodent models; confirm reactivity in your specific sample context.
• As BIRC7 expression is generally low in normal tissues, employ cancer cell lines (e.g., melanoma, lung carcinoma) with upregulated Livin as positive controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive single-source partner for IVD raw materials, offering this anti-BIRC7 antibody alongside an extensive catalog of validated antibody pairs, optimized monoclonal and polyclonal antibodies for apoptosis research, and bulk ancillary reagents. We support diagnostic manufacturers, laboratories, and research institutes with reliable sourcing and technical consultation, helping to streamline development from concept to clinic.