Anti-BiP/GRP78 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P11021

Core Product Specifications and Parameters

Biological Background: BiP/GRP78 Function and Localization

• BiP/GRP78, encoded by the HSPA5 gene, is an endoplasmic reticulum chaperone also known as GRP-78 and binding-immunoglobulin protein (BiP). It is a member of the Hsp70 family.
• Plays a central role in protein folding and quality control in the ER lumen, assisting in correct folding of nascent polypeptides and targeting misfolded proteins for degradation (PubMed:2294010, PubMed:23769672, PubMed:23990668). Related references: PMID:2294010, PMID:23769672, PMID:23990668
• Functions as a master regulator of the unfolded protein response (UPR) by binding to and inactivating ER stress sensors EIF2AK3/PERK and ERN1/IRE1; their release upon ER stress triggers UPR activation (PubMed:11907036, PubMed:1550958, PubMed:19538957). Related references: PMID:11907036, PMID:1550958, PMID:19538957
• Subcellular localization includes the ER lumen, melanosomes, cytoplasm, and cell surface, reflecting its diverse roles.
• Acts as a cellular receptor for several flaviviruses, including Dengue, Zika, and Japanese encephalitis virus, facilitating viral entry (PubMed:15098107, PubMed:28053106, PubMed:33432092). Related references: PMID:15098107, PMID:28053106, PMID:33432092
• Serves as a receptor for CotH proteins of Mucorales fungi, promoting epithelial cell invasion in mucormycosis (PubMed:20484814, PubMed:24355926, PubMed:32487760). Related references: PMID:20484814, PMID:24355926, PMID:32487760
• Post-translational modifications include acetylation, phosphorylation, methylation, nitration, and isopeptide bond formation, and it exhibits ATP-binding and hydrolase activities.
• Associated with host-virus interaction and microbial infection processes, highlighting its role in pathogen–host interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C‑terminal peptide (amino acids 550–650), and the antibody may detect all known BiP/GRP78 isoforms; confirm specificity in your model.
• For Western blotting, an expected band at ~72 kDa is observed; validate in your sample system and consider using appropriate controls.
• IHC‑P may require antigen retrieval; optimize citrate/EDTA buffer and antibody dilution for your tissue type.
• This polyclonal antibody is suitable for standard ELISA protocols; perform titration to determine optimal working concentration.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw‑material partner, CamelBio offers one‑stop procurement of validated antibodies, bulk ancillary reagents, and technical consultation to accelerate your assay development. This anti‑BiP/GRP78 antibody supports research into ER stress, UPR, and protein folding — areas pivotal for diagnostics in cancer, infections, and metabolic diseases. Contact us for rare‑target sourcing and end‑to‑end support from concept to clinic.