Anti-Biotinidase (BTD) Polyclonal Antibody for WB, IHC-P, ELISA - P43251

Core Product Specifications and Parameters

Biological Background: Biotinidase Function and Localization

• Biotinidase (BTD) is a secreted enzyme that catalyzes the release of biotin from biocytin, a degradation product of biotin-dependent carboxylases.
• The enzyme is crucial for biotin recycling and maintaining free biotin pools, essential for carboxylase activities in fatty acid synthesis, gluconeogenesis, and amino acid catabolism.
• Biotinidase is secreted into the extracellular space, where it acts on biocytin derived from proteolytic degradation of carboxylases.
• The BTD gene undergoes alternative splicing, producing multiple transcript variants; the protein is a glycoprotein as indicated by post-translational modifications.
• Mutations in BTD are associated with biotinidase deficiency, a disorder characterized by neurological and cutaneous symptoms due to impaired biotin recycling.
• The enzyme belongs to the nitrilase/cyanide hydratase superfamily and functions as a hydrolase, cleaving the amide bond of biocytin.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (residues 284-543) of human biotinidase, which may allow detection of full-length protein and certain isoforms harboring this segment.
• For Western blot (WB), a band around 61 kDa is expected based on the immunogen; note that glycosylation and alternative splicing can lead to minor size variations.
• For immunohistochemistry (IHC-P), antigen retrieval conditions and tissue fixation should be optimized; validate in liver or kidney sections where biotinidase is known to be produced.
• In ELISA, the antibody can be utilized for capture or detection; confirm reactivity against recombinant biotinidase or native protein in biological fluids.
• Cross-reactivity with mouse and rat has been reported; however, perform species-specific validation when switching model systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive supply partner for diagnostic manufacturers and research laboratories, offering this anti-BTD polyclonal antibody as part of a broader portfolio of IVD raw materials. This product supports studies in biotin metabolism and biotinidase deficiency, and we can facilitate sourcing of validated antibody pairs, bulk ancillary reagents, and rare-target raw materials tailored to your development pipeline. With end-to-end capabilities from concept to clinic, CamelBio ensures reliable access to critical reagents for your biotin-related assay development.