Anti-BIN3 Rabbit Polyclonal Antibody for WB, ELISA - Q9NQY0

Core Product Specifications and Parameters

Biological Background: BIN3 Function and Localization

• BIN3 (Bridging integrator 3) is involved in cytokinesis and septation, where it plays a role in the localization of F-actin.
• It localizes to the cytoplasm and associates with the cytoskeleton.
• BIN3 is ubiquitously expressed in human tissues, with the exception of brain.
• The protein undergoes alternative splicing and contains a coiled-coil domain; it is associated with cell cycle and cell division processes.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1–253 of human BIN3. As a polyclonal antibody, it may recognize multiple epitopes, offering robust detection in WB and ELISA.
• For WB, the expected molecular weight is ~30 kDa. Consider optimizing the primary antibody dilution using a titration series with appropriate positive control lysates (e.g., from human, mouse, or rat cells).
• In ELISA, test a range of coating antigen concentrations and antibody dilutions to determine optimal signal-to-noise ratios.
• Because BIN3 functions in cytokinesis, synchronization of cell cultures may enhance detection in proliferation-related experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a seamless supply of IVD raw materials, from development to commercialization. This anti-BIN3 antibody is part of our comprehensive portfolio for cell biology targets, supporting cytokinesis and cytoskeleton research. We also offer custom sourcing for validated antibody pairs, bulk ancillary reagents, and rare target antigens to accelerate your assay development.