Anti-BIN1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O00499

Core Product Specifications and Parameters

Biological Background: BIN1 Function and Localization

• BIN1 (Myc box-dependent-interacting protein 1), also known as Amphiphysin II, is a member of the amphiphysin/BIN family. It contains an SH3 domain and coiled-coil regions, and undergoes alternative splicing to generate tissue-specific isoforms (e.g., brain-specific isoform IIA).
• Functions as a key regulator of plasma membrane curvature and remodeling, essential for T-tubule formation in muscle cells (PubMed:24755653). Related references: PMID:24755653
• Acts as a negative regulator of endocytosis and is involved in intracellular vesicle sorting, including modulation of BACE1 trafficking and amyloid-beta production (PubMed:27179792). In neurons, this may influence internalization of tau aggregates. Related references: PMID:27179792
• May regulate MYC activity and cell proliferation through direct interaction (PubMed:8782822). Related references: PMID:8782822
• Exhibits actin bundling activity and stabilizes actin filaments against depolymerization in vitro (PubMed:28893863). Related references: PMID:28893863
• Subcellularly, BIN1 localizes to the nucleus, cytoplasm, endosomes, and plasma membrane, including the sarcolemma and T-tubules in muscle cells.
• Ubiquitously expressed with highest levels in brain and muscle; isoform IIA is restricted to brain gray matter.

Experimental Guidance and Technical Tips

• For western blotting, expect a band at approximately 65 kDa. Validate detection in relevant sample systems (e.g., brain, muscle, or cell lines).
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize antigen retrieval and include appropriate negative controls to confirm tissue-specific staining.
• The immunogen (aa 230–409) covers a region shared by several isoforms; consider verifying isoform specificity if differential expression is critical to the study.
• As a polyclonal antibody, titration is recommended for each new application and lot. Cross-reactivity with rat is confirmed; homology of the immunogen sequence should be checked for other species.

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