Anti-BIN1 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O00499

Core Product Specifications and Parameters

Biological Background: BIN1 Function and Localization

• BIN1 (Myc box-dependent-interacting protein 1), also known as Amphiphysin II, is encoded by the BIN1 gene and produces multiple isoforms via alternative splicing, including the brain-specific isoform IIA and the ubiquitously expressed isoform BIN1 that is highest in skeletal muscle.
• It is a key regulator of plasma membrane curvature, shaping, and remodeling, required for T-tubule formation in muscle cells and acting as a negative regulator of endocytosis. (PubMed:24755653)
  Related references:
  PMID:24755653
• BIN1 modulates intracellular vesicle sorting and BACE1 trafficking, thereby controlling amyloid-beta production, linking it to Alzheimer's disease pathology. (PubMed:27179792)
  Related references:
  PMID:27179792
• BIN1 may regulate MYC activity and cell proliferation. (PubMed:8782822)
  Related references:
  PMID:8782822
• BIN1 has actin bundling activity and stabilizes actin filaments against depolymerization in vitro. (PubMed:28893863)
  Related references:
  PMID:28893863
• Subcellularly, BIN1 localizes to the nucleus, cytoplasm, endosome, cell membrane, sarcolemma, and T-tubules, consistent with its roles in membrane dynamics and signaling.
• BIN1 is ubiquitously expressed, with highest levels in brain and muscle; it is also detected in oligodendrocytes. Isoform IIA is brain-specific, while isoform BIN1 is widely expressed, peaking in skeletal muscle. (PubMed:9182667, PubMed:27488240)
  Related references:
  PMID:9182667
  PMID:27488240
• Keywords include alternative splicing, cell membrane, coiled coil, SH3 domain, phosphoprotein, and involvement in host-virus interaction, developmental processes, and differentiation, underscoring its multifunctional nature.

Experimental Guidance and Technical Tips

• The immunogen consists of a recombinant fusion protein spanning amino acids 511–593 of human BIN1 (NP_647593.1), which corresponds to the C-terminal region containing the SH3 domain. This design may enable recognition of multiple BIN1 isoforms and is suitable for detecting both endogenous and overexpressed protein.
• For Western blotting (WB), the predicted molecular weight is approximately 65 kDa, but isoforms and post-translational modifications may produce additional bands; validate the antibody with positive and negative controls using relevant cell or tissue lysates.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), antigen retrieval and permeabilization conditions should be optimized, especially for membranous or cytoskeletal targets, to ensure clear localization patterns.
• The antibody is recommended for ELISA; consider a sandwich ELISA format with proper matched antibody pairs for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material sourcing for diagnostic manufacturers, laboratories, and research institutions by offering a comprehensive portfolio of validated antibodies, antigens, and ancillary reagents. This anti-BIN1 rabbit monoclonal antibody supports neuroscience and muscle biology research, targeting a critical regulator of membrane dynamics. Through our integrated supply chain and technical consulting, we ensure reliable access to high-quality raw materials, from concept to clinic.