Anti-BIN1 Monoclonal Antibody for WB, IHC-P, ELISA - O00499

Core Product Specifications and Parameters

Biological Background: BIN1 Function and Localization

• BIN1 (Myc box-dependent-interacting protein 1), also known as Amphiphysin II, is a key regulator of plasma membrane curvature and remodeling, essential for T-tubule formation in muscle cells. Related references: PMID:24755653
• It acts as a negative regulator of endocytosis and is involved in intracellular vesicle sorting and BACE1 trafficking, influencing amyloid-beta production. Related references: PMID:27179792
• BIN1 may regulate endocytosis in neuronal circuits, affecting the internalization of PHF-tau aggregates.
• It is involved in regulating MYC activity and cell proliferation. Related references: PMID:8782822
• BIN1 possesses actin bundling activity and stabilizes actin filaments against depolymerization in vitro. Related references: PMID:28893863
• Subcellularly, BIN1 localizes to the nucleus, cytoplasm, endosomes, cell membrane, sarcolemma, and T-tubules.
• Tissue expression is ubiquitous, with highest levels in brain and muscle; isoform IIA is brain-specific, and the major isoform is highly expressed in skeletal muscle.
• Key features include alternative splicing, a coiled-coil domain, an SH3 domain, and roles in development, differentiation, and host-virus interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 400–593, the C-terminal region containing the SH3 domain. This design targets a functional domain, potentially recognizing multiple isoforms.
• For Western blot, BIN1 is predicted at ~65 kDa; validate the observed band size in your sample lysates, considering possible posttranslational modifications or isoform variants.
• For IHC-P, consider optimizing antigen retrieval and antibody dilution using tissues with known high expression (e.g., brain, skeletal muscle).
• For ELISA, use recombinant BIN1 protein as a standard to assess binding affinity and specificity.
• Cross-reactivity is reported for human, mouse, and rat; verify reactivity in other species if needed.
• This antibody is provided in a format suitable for routine applications; for sandwich immunoassays, evaluate as a capture or detection antibody in combination with a suitable pair.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes with a comprehensive range of IVD raw materials, technical services, and consulting—from concept to clinic. Our portfolio includes validated monoclonal antibodies, recombinant proteins, and ancillary reagents for membrane trafficking and neurodegenerative disease research. For BIN1-related projects, we can help source rare targets, optimized antibody pairs, and bulk reagents to accelerate your assay development and scale-up.