Anti-Bim Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O43521

Core Product Specifications and Parameters

Biological Background: Bim (BCL2L11) Function and Localization

• Bim (Bcl-2-like protein 11, encoded by BCL2L11) is a pro-apoptotic member of the Bcl-2 protein family that induces apoptosis and anoikis.
• Multiple isoforms arise from alternative splicing, with BimEL, BimL, and BimS being the predominant and most potent apoptosis inducers; isoform BimL is more potent than BimEL.
• Additional isoforms such as Bim-alpha1, Bim-alpha2, Bim-alpha3, and Bim-gamma also promote apoptosis, while BimAC and BimABC lack pro-apoptotic activity.
• Bim localizes predominantly to the mitochondrial outer membrane and endomembrane system, where it interacts with other Bcl-2 family members to regulate intrinsic cell death.
• The protein is widely expressed with tissue-specific variation; Bim-gamma is most abundant in small intestine and colon, and present at lower levels in spleen, prostate, testis, heart, liver, and kidney.
• Post-translational modifications include phosphorylation and ubiquitination (Ubl conjugation), which control Bim stability and apoptotic activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (amino acids 100–198) of human Bim, potentially recognizing multiple isoforms. For Western blot, anticipate a primary band around 22 kDa, with possible isoform-specific variations.
• Validate antibody specificity by including appropriate controls (e.g., knockdown or knockout lysates) and observing expected size shifts under reducing conditions.
• For immunofluorescence (IF/ICC), fix and permeabilize cells according to standard protocols and titrate the antibody to minimize background; confirm co-localization with mitochondrial markers.
• When using this antibody in ELISA, pair it with a suitable detection system and test serial dilutions to establish optimal working concentrations for your sample type.

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CamelBio supports diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio of IVD raw materials, technical services, and consulting from concept to clinic. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies like this anti-Bim reagent, bulk ancillary reagents, and sourcing for rare-target raw materials. Whether you need reliable apoptosis-related antibodies or customized supply solutions, our one-stop platform bridges your procurement and development needs.