Anti-Bim Rabbit Polyclonal Antibody for IHC-P and ELISA - O43521

Core Product Specifications and Parameters

Biological Background: Bim Function and Localization

• Bim (Bcl-2-like protein 11) is a pro-apoptotic BH3-only member of the Bcl-2 protein family, encoded by the BCL2L11 gene. Multiple isoforms (BimEL, BimL, BimS, Bim-alpha1, etc.) are generated via alternative splicing.
• Induces apoptosis and anoikis through its BH3 domain, which binds and neutralizes anti-apoptotic Bcl-2 proteins. Isoform BimL is more potent than BimEL, and BimS is the most potent. Isoforms Bim-alpha1/2/3 also induce apoptosis but less efficiently, while BimAC and BimABC lack apoptotic activity.
• Localizes to the endomembrane system and the outer mitochondrial membrane, where it mediates cytochrome c release by activating BAX/BAK.
• Widely expressed, with BimEL, BimL, and BimS as predominant isoforms showing tissue-specific variation. Isoform Bim-gamma is most abundant in small intestine and colon.
• Subject to post-translational modifications: phosphorylation regulates protein stability and binding affinity to pro-survival partners, and ubiquitination targets Bim for proteasomal degradation.
• Structural studies highlight the critical role of the BH3 amphipathic α-helix in receptor binding and the C-terminal hydrophobic tail in membrane insertion.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 30–130 of human Bim (NP_619527.1), a region shared by multiple isoforms. Confirm isoform specificity if required.
• For IHC-P, consider standard antigen retrieval methods (e.g., citrate buffer, pH 6.0) and validate staining with appropriate positive (e.g., lymphoid tissue) and negative controls.
• For ELISA, assess reactivity against the full-length recombinant immunogen or relevant isoform proteins, and include a non-specific binding control.
• Western blot detection may be possible but is not guaranteed; if attempted, note that the predicted molecular weight of Bim isoforms ranges from ~16–23 kDa, and endogenous expression can be low in unstimulated cells.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw material procurement, covering the entire diagnostic development pipeline. This anti-Bim polyclonal antibody supports apoptosis and cell death research applications, and we also supply validated antibody pairs, optimized recombinant proteins, and ancillary reagents. For rare or custom targets, our sourcing network can help secure the critical raw materials you need to advance your project.