Anti-Bim Rabbit Monoclonal Antibody for WB and ELISA - O43521

Core Product Specifications and Parameters

Biological Background: Bim Function and Localization

• Bim (Bcl-2-like protein 11, also known as Bcl2-interacting mediator of cell death) is a pro-apoptotic member of the Bcl-2 family, encoded by the BCL2L11 gene. Multiple isoforms exist due to alternative splicing, including BimEL, BimL, BimS, and others, with varying apoptotic potencies.
• Isoforms BimL and BimS are the most potent inducers of apoptosis and anoikis, while BimEL, Bim-alpha1/2/3, and Bim-gamma also promote cell death but with reduced efficacy. Isoforms BimAC and BimABC lack apoptotic activity.
• Bim plays a critical role in mitochondrial-mediated apoptosis by neutralizing anti-apoptotic Bcl-2 family members, facilitating cytochrome c release and caspase activation.
• Subcellularly, Bim localizes primarily to the endomembrane system and mitochondria, consistent with its function in apoptosis regulation.
• Post-translational modifications include phosphorylation and ubiquitination (Ubl conjugation), which regulate Bim stability and activity. The protein's 3D structure has been studied.
• Tissue-specific expression varies: BimEL, BimL, and BimS are widely expressed, while Bim-gamma is most abundant in small intestine and colon, with lower levels in spleen, prostate, testis, heart, liver, and kidney.
• As a key apoptosis regulator, Bim is involved in development, homeostasis, and disease, including cancer and neurodegenerative disorders.

Experimental Guidance and Technical Tips

• The immunogen for this antibody is a synthetic peptide spanning amino acids 1-100 of human Bim (O43521). This N-terminal region is shared among multiple isoforms, suggesting the antibody may detect several Bim variants. Confirm isoform specificity in your experimental system.
• For Western blot (WB), the predicted molecular weight of the canonical Bim isoform is ~22 kDa (UniProt: 22,171 Da). Observe banding patterns around this size, considering possible alternative isoforms or post-translational modifications.
• The antibody has been validated for ELISA and WB applications with cross-reactivity to human, mouse, and rat. When using in ELISA, optimize coating and detection conditions for your specific assay format.
• Since Bim expression can be induced by various stress signals, consider appropriate positive and negative controls (e.g., untreated vs. apoptotic cells) to validate antibody performance.
• For quantitative studies, be aware that Bim turnover is regulated by phosphorylation and ubiquitination; signal intensity may vary with cellular context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering manufacturers and laboratories validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-Bim antibody supports apoptosis research and can be integrated into diagnostic assays targeting Bcl-2 family biomarkers. For projects requiring rare-target raw materials or full development support from concept to clinic, CamelBio provides streamlined procurement and technical consultation.