Anti-Bif-1 Rabbit Polyclonal Antibody for IF/ICC, ELISA - Q9Y371

Core Product Specifications and Parameters

Biological Background: Bif-1 Function and Localization

• Endophilin-B1 (Bif-1, SH3GLB1) is a 41 kDa protein encoded by the SH3GLB1 gene in humans, existing as two alternatively spliced isoforms with distinct roles: isoform 1 promotes apoptosis, while isoform 2 protects neuronal viability.
• Bif-1 plays a critical role in mitochondrial outer membrane dynamics and is required for normal mitochondrial morphology and function.
  Related references: PMID:15452144
• It is a key mediator of caspase-dependent apoptosis by promoting BAX/BAK1 activation and is also involved in caspase-independent cell death during nutrient starvation.
  Related references: PMID:16227588
• Bif-1 activates the lipid kinase PIK3C3 (VPS34) during autophagy by associating with the PI3K complex II (PI3KC3-C2), regulating autophagosome formation and ATG9A trafficking from the Golgi.
  Related references: PMID:17891140 PMID:21068542
• It participates in membrane remodeling events, including Golgi membrane tubulation and fragmentation, and may promote membrane fusion by recruiting proteins to high-curvature membranes.
  Related references: PMID:11604418
• Subcellularly, Bif-1 localizes to the cytoplasm, Golgi apparatus, mitochondrial outer membrane, autophagosome membrane, and the midbody during cytokinesis.
• Tissue expression is highest in heart, skeletal muscle, kidney, and placenta, with lower levels in brain, colon, thymus, spleen, liver, small intestine, lung, and peripheral blood leukocytes.
• Post-translationally, Bif-1 is subject to acetylation and phosphorylation; it features an SH3 domain and coiled-coil regions critical for protein interactions and membrane association.

Experimental Guidance and Technical Tips

• For IF/ICC, use appropriate fixation (e.g., 4% PFA) and permeabilization (e.g., 0.1% Triton X-100). Consider including a blocking step with 5% BSA to reduce background.
• In ELISA, perform a dilution series (e.g., starting from 1:500) to determine the optimal antibody concentration for your specific antigen coating and detection system.
• Validate antibody specificity in human cell lines known to express Bif-1, such as HeLa or HEK293. Include a negative control (e.g., siRNA knockdown) to confirm target recognition.
• Since this is a polyclonal antibody, be aware of potential batch-to-batch variability; we recommend qualifying each new lot in your assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. This Bif-1 antibody supports research in apoptosis, autophagy, and mitochondrial dynamics, and our team can assist with rare target sourcing and custom development to accelerate your diagnostic or research programs.