Anti-Bid Rabbit mAb (KD Validated) for WB, ELISA - P55957

Core Product Specifications and Parameters

Biological Background: Bid Function and Localization

• Bid, also known as p22 BID, is a BH3-interacting domain death agonist that promotes caspase activation and apoptosis. Related references: PMID:14583606
• Upon activation, Bid translocates to mitochondria and disrupts the outer mitochondrial membrane, triggering cytochrome c release. Related references: PMID:10480954, PMID:32029622, PMID:9727491
• Bid is primarily cytoplasmic in its inactive form, but following cleavage, the truncated form (tBid) localizes to the mitochondrial outer membrane.
• Bid is expressed in tissues such as spleen, pancreas, placenta, and lung at the protein level.
• Post-translational modifications, including acetylation, phosphorylation, and ubiquitination, regulate Bid's activity and stability.
• Alternative splicing generates isoforms that may have different apoptotic functions.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1-195 of human Bid, representing the full-length protein. This antibody may recognize both full-length Bid (~22 kDa) and cleaved fragments upon apoptosis induction.
• For Western blotting, consider using apoptosis-inducing agents to detect tBid and validate the relevant band pattern in your sample system.
• For ELISA, a full-length recombinant Bid protein is recommended as the coating antigen to ensure proper detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio equips diagnostic manufacturers and research institutes with a one-stop supply of IVD raw materials, including validated antibody pairs and optimized monoclonal antibodies like this anti-Bid clone. For projects focused on mitochondrial apoptosis markers, we offer sourcing of rare targets, bulk ancillary reagents, and technical support to accelerate your assay development from concept to clinic.