Anti-BHMT Rabbit Polyclonal Antibody for WB, IF-P, ELISA - Q93088

Core Product Specifications and Parameters

Biological Background: BHMT Function and Localization

• BHMT (Betaine--homocysteine S-methyltransferase 1) is a zinc metalloenzyme that catalyzes the transfer of a methyl group from betaine to homocysteine, producing dimethylglycine and methionine. This reaction is central to homocysteine homeostasis and is necessary for the irreversible oxidation of choline.
• The protein is also designated BHMT1, HEL-S-61p, and by its official symbol BHMT.
• Expression is highly tissue-specific, occurring almost exclusively in the liver and kidney.
• At the subcellular level, BHMT is detected in the cytoplasm, cytosol, and nucleus.
• Post-translational modifications include phosphorylation (as indicated by the keyword "Phosphoprotein"), and the enzyme requires zinc for catalytic activity.
• The predicted molecular weight of the unprocessed precursor is approximately 45 kDa (44998 Da).

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 177–406 of human BHMT isoform 1, a region likely to generate polyclonal antibodies with multiple epitope specificities. Consider validating specificity by pre-adsorption with the recombinant immunogen.
• For Western blot, a band migrating at ~45 kDa is expected. Optimize antibody dilution using positive controls such as liver or kidney lysates, which naturally express BHMT.
• In immunofluorescence (IF-P), both cytoplasmic and nuclear staining may be observed; fixation and permeabilization protocols should be optimized for your cell type.
• The antibody can be adapted for ELISA-based assays; for quantitative detection, pairing with a well-characterized detection antibody is advised.
• As a polyclonal reagent, lot-to-lot variability is possible; always confirm performance in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio stands as a comprehensive IVD raw-material partner for diagnostic manufacturers, laboratories, and research institutes, offering end-to-end support from concept to clinic. This anti-BHMT antibody serves research into homocysteine metabolism, liver and kidney function, and related disorders. Leverage CamelBio’s supply capabilities for validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target sourcing to accelerate your assay development and procurement workflows.