Anti-BHLHE41 Rabbit Polyclonal Antibody for WB and ELISA - Q9C0J9

Core Product Specifications and Parameters

Biological Background: BHLHE41 Function and Localization

• BHLHE41 (also known as DEC2, SHARP1, bHLHb3) is a basic helix-loop-helix transcription factor that functions as a transcriptional repressor, primarily involved in the regulation of circadian rhythms by negatively controlling clock gene expression. Related references: PMID:11278948, PMID:14672706, PMID:15193144

• It forms a negative autoregulatory feedback loop (DEC loop), distinct from the PER/CRY loop, and represses its own expression as well as that of DBP and PER1; both loops interlock to maintain circadian oscillations. Related references: PMID:11278948, PMID:14672706, PMID:15193144

• BHLHE41 represses the transcriptional activity of the CLOCK-BMAL1 heterodimer by competing for E-box elements (5'-CACGTG-3') in target gene promoters, thereby attenuating circadian output. Related references: PMID:25083013

• Beyond circadian regulation, BHLHE41 acts as a corepressor of nuclear receptors such as RXR, LXR, and VDR, inhibiting ligand-induced transactivation (By similarity).

• The protein localizes predominantly to the nucleus, consistent with its role as a DNA-binding transcriptional regulator.

• BHLHE41 is subject to post-translational modifications, including isopeptide bond formation and ubiquitin conjugation (Ubl conjugation), which may regulate its stability or activity.

• It is highly expressed in skeletal muscle and brain, moderately in pancreas and heart, and weakly in placenta, lung, liver, and kidney, indicating tissue-specific regulatory functions.

Experimental Guidance and Technical Tips

• Given the immunogen covers amino acids 120-280, consider that the antibody may recognize both human and rat BHLHE41, as indicated by the cross-reactivity data.
• For Western blotting, a band around 50 kDa is expected; validate using appropriate positive controls.
• For ELISA, the antibody can be used for detection of recombinant or endogenous BHLHE41; consider optimizing the coating antigen and detection system based on the specific assay format.

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CamelBio is your one-stop partner for sourcing high-quality IVD raw materials, from validated antibody pairs and optimized polyclonal antibodies to bulk ancillary reagents and rare-target custom formulations. Whether you are developing diagnostics for metabolic or chronobiology applications, CamelBio streamlines procurement for BHLHE41-related reagents and beyond. Explore our full portfolio to accelerate your assay development pipelines.