Anti-BHLHE40 Polyclonal Antibody for WB, IHC-P, ELISA - O14503

Core Product Specifications and Parameters

Biological Background: BHLHE40 Function and Localization

• BHLHE40 is a Class E basic helix-loop-helix transcriptional repressor that plays a critical role in circadian rhythm regulation by negatively regulating clock genes and clock-controlled genes. Related references: PMID:12397359, PMID:18411297
• It acts as the negative limb of an autoregulatory feedback loop (DEC loop) distinct from the PER/CRY loop, repressing PER1/2 and being itself repressed by PER1/2 and CRY1/2. Related references: PMID:14672706, PMID:15193144
• Represses circadian transcriptional activators by competing with CLOCK-BMAL1 heterodimers for E-box binding in target gene promoters. Related references: PMID:15560782
• Negatively regulates its own expression as well as DBP and BHLHE41/DEC2. Related references: PMID:14672706
• Functions as a corepressor for RXR and RXR-LXR heterodimers, inhibiting ligand-induced transactivation of RXRA and NR1H3/LXRA. Related references: PMID:19786558
• Involved in chondrocyte differentiation via the cAMP pathway and contributes to circadian blood pressure control by repressing ATP1B1 transcription. Related references: PMID:19786558, PMID:30012868
• Subcellular localization includes both cytoplasm and nucleus, consistent with its transcriptional regulatory role.
• Broadly expressed in tissues including cartilage, spleen, intestine, lung, heart, brain, liver, muscle, and stomach.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 121–300, a region that may include functional domains such as the bHLH domain and the Orange domain; consider verifying epitope specificity with truncated constructs if needed.
• For western blot, the predicted molecular weight is ~46 kDa; however, post-translational modifications may cause a shift, so validate using positive control lysates from tissues like cartilage or spleen.
• For immunohistochemistry (IHC-P), antigen retrieval conditions should be optimized; test on known expressing tissues and include a nuclear staining control.
• Cross-reactivity with mouse and rat suggests suitability for these species, but always confirm with appropriate controls in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. For circadian rhythm and transcription research, CamelBio offers BHLHE40 antibodies and related molecular tools to support assay development and rare target sourcing, streamlining your path from concept to clinical application.