Anti-BGN Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P21810

Core Product Specifications and Parameters

Biological Background: Biglycan Function and Localization

• Biglycan (BGN) is a small leucine-rich proteoglycan (SLRP) and is also known as bone/cartilage proteoglycan I (PG-S1).
• It may be involved in collagen fiber assembly, highlighting its role in extracellular matrix (ECM) organization.
• BGN belongs to the SLRP family, which is characterized by leucine-rich repeat motifs important for protein interactions.
• The protein is secreted and predominantly localizes to the extracellular space and ECM.
• Biglycan is detected in placenta and other connective tissues, with high expression in articular cartilages. Related references: PMID:32337544
• As a proteoglycan, biglycan is glycoprotein and contains disulfide bonds, contributing to its structural stability.
• Mutations in the BGN gene have been associated with disorders such as aortic aneurysm and dwarfism, underscoring its clinical relevance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the mature biglycan sequence (aa 38-368), which lacks the signal peptide, ensuring recognition of the processed protein.
• For Western blot (WB), consider using protein extracts from ECM-rich tissues (e.g., cartilage, placenta) to detect the ~42 kDa band; glycosylation may cause a shift in apparent molecular weight.
• In immunofluorescence (IF/ICC), expect perinuclear/cellular matrix staining; optimize permeabilization and fixation protocols for your sample.
• Cross-reactivity with mouse is reported; validate in your specific experimental system.
• As a polyclonal antibody, it may detect multiple glycosylated variants; for quantitative ELISA, use an appropriate recombinant protein standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to high-quality IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our portfolio for extracellular matrix and connective tissue research targets like biglycan supports assay development from concept to clinic. Whether you require optimized antibodies or specialized rare-target reagents, CamelBio streamlines your procurement with end-to-end technical support.