Anti-BFAR Polyclonal Antibody for WB, IF/ICC, ELISA - Q9NZS9

Core Product Specifications and Parameters

Biological Background: BFAR Function and Localization

• BFAR (Bifunctional apoptosis regulator), also known as RING finger protein 47 (RNF47), is a membrane-bound E3 ubiquitin ligase. Alternative splicing generates multiple isoforms, contributing to functional diversity.
• It exhibits anti-apoptotic activity, suppressing cell death triggered by both death-receptors and mitochondrial factors. Related references: PMID:14502241
• During endoplasmic reticulum (ER) stress, BFAR controls IRE1/ERN1 signaling by promoting ubiquitin-proteasome degradation of the inhibitor TMBIM6. Related references: PMID:21068390
• BFAR activates TGF-beta signaling by catalyzing 'Lys-63'-linked ubiquitination of the TGFBR1 receptor, a critical step for pathway initiation. Related references: PMID:33914044
• In concert with NGFR, BFAR negatively regulates NF-kappa-B and JNK signaling pathways, influencing cellular stress responses. Related references: PMID:22566094
• BFAR mediates the proteasomal degradation of PNPLA3, a key player in lipid metabolism, linking it to hepatic lipid homeostasis. Related references: PMID:38294943
• The protein is anchored to the endoplasmic reticulum membrane, where it exerts its ubiquitin ligase functions and interacts with stress-related substrates.
• Tissue distribution analysis reveals high BFAR expression in the brain, moderate in the small intestine, weak in testes and liver, and absence in heart, kidney, lung, and spleen. It is characterized as a glycoprotein with zinc-finger domains, metal-binding, and transferase activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-140) of human BFAR, which may allow recognition of multiple isoforms if this region is conserved. Validate isoform specificity in your model system.
• For Western blot (WB), the predicted molecular weight is 53 kDa; however, due to post-translational modifications, the observed band may differ. Consider using appropriate positive controls (e.g., brain or small intestine lysates).
• When performing immunofluorescence/immunocytochemistry (IF/ICC), BFAR localizes to the endoplasmic reticulum; co-staining with an ER marker is recommended to confirm specificity.
• For ELISA, ensure optimal antibody dilution and antigen coating conditions for your specific assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-BFAR polyclonal antibody, targeting apoptosis and ER stress pathways, is part of our extensive portfolio of validated antibodies. We supply optimized antibody pairs, bulk ancillary reagents, and can source rare target raw materials to support your IVD assay development.