Anti-Beta-galactosidase (GLB1) Rabbit Polyclonal Antibody for WB, ELISA - P16278

Core Product Specifications and Parameters

Biological Background: Beta-galactosidase Function and Localization

• GLB1 encodes beta-galactosidase, a lysosomal enzyme that removes terminal beta-linked galactose residues from substrates such as gangliosides, glycoproteins, and glycosaminoglycans.
• The protein also functions as the elastin receptor 1 (EBP), a non-catalytic chaperone that binds tropoelastin and facilitates its secretion and assembly into elastic fibers during elastogenesis.
• Subcellular localization includes lysosomes and the perinuclear cytoplasm.
• Tissue expression is detected in placenta (at protein level) (PubMed:8383699), and in fibroblasts and testis (PubMed:2511208). Related references: PMID:8383699, PMID:2511208
• Post-translational modifications include glycosylation and disulfide bond formation, with the protein existing as a zymogen requiring activation.
• Mutations in GLB1 are associated with lysosomal storage diseases, including GM1 gangliosidosis and mucopolysaccharidosis type IVB (Morquio B syndrome).
• Alternative splicing generates multiple protein isoforms that may have distinct functional roles.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-270), which may recognize multiple GLB1 isoforms; consider sequence alignment for isoform specificity.
• For Western blot, the predicted molecular weight is ~76 kDa; validate band detection with appropriate positive and negative control lysates.
• This antibody is reactive with human, mouse, and rat samples; cross-species validation is recommended for non-verified species.
• ELISA applications may require optimization of coating antigen and antibody dilutions depending on the assay format.
• Due to alternative splicing and dual functionality, confirm detection of the target in the relevant cellular compartment (lysosome vs. cytoplasm/perinuclear region) using fractionation or co-localization studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research labs, and institutes with a one-stop supply channel for IVD raw materials, simplifying procurement from target selection to production. This GLB1 antibody is part of our extensive portfolio covering lysosomal storage diseases and elastic fiber biology, supported by validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and sourcing of rare-target materials. Contact our team for tailored procurement solutions to accelerate your assay development.