Anti-beta 2 Microglobulin Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P61769

Core Product Specifications and Parameters

Biological Background: beta 2 Microglobulin Function and Localization

• Beta-2-microglobulin (B2M) is the light chain component of the class I major histocompatibility complex (MHC), essential for the presentation of peptide antigens to the immune system.
• Exogenously applied M. tuberculosis EsxA or EsxA-EsxB (or host-expressed EsxA) binds B2M and reduces its cell surface export, potentially impairing class I antigen presentation. Related references: PMID:25356553
• B2M belongs to the immunoglobulin superfamily and features a classical immunoglobulin domain structure.
• The protein is localized to the cell surface as part of MHC I complexes, and it is also secreted.
• Post-translational modifications include glycation, glycosylation, a conserved disulfide bond, and N-terminal pyrrolidone carboxylic acid formation.
• B2M is associated with amyloidosis (amyloidogenic forms) and has been linked to disease variants.
• It is a key molecule in adaptive immunity, serving as a reference proteome member identified through proteomics.

Experimental Guidance and Technical Tips

• The immunogen consists of a recombinant fusion protein corresponding to amino acids 21-119 of human beta 2 Microglobulin (NP_004039.1). This region includes the mature protein sequence, excluding the signal peptide, and is expected to generate antibodies recognizing the full-length native protein.
• For Western blot (WB), the predicted molecular weight is ~14 kDa. Ensure proper protein loading controls and membrane blocking to minimize background.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), antigen retrieval may be necessary; optimization of fixation and permeabilization conditions is recommended.
• Given its polyclonal nature, lot-to-lot consistency should be verified for critical assays; screening by ELISA or dot blot is advisable.
• Cross-reactivity with mouse and rat orthologs is reported, but sequence alignment and pilot experiments are recommended to confirm binding in these species.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio connects diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio of IVD raw materials. As a specialized partner, we offer validated polyclonal and monoclonal antibodies, matched antibody pairs, and ancillary reagents to streamline your assay development. For researchers focused on immune function and MHC I pathways, this anti-beta 2 Microglobulin antibody is a reliable choice, and we can support bulk orders, custom conjugation, or sourcing of rare targets.