Anti-beta 2 Microglobulin (B2M) Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, FC, ELISA - P61769

Core Product Specifications and Parameters

Biological Background: Beta-2-microglobulin Function and Localization

• Beta-2-microglobulin (B2M) is the light chain of the class I major histocompatibility complex (MHC), essential for presenting peptide antigens to the immune system. Exogenously applied M.tuberculosis EsxA decreases B2M export to the cell surface, potentially impairing class I antigen presentation. Related references: PMID:25356553
• It is secreted and displayed on the cell surface as a component of MHC I, bridging antigen-loaded heavy chains and CD8+ T cells.
• Structurally, B2M contains an immunoglobulin-like domain and a conserved disulfide bond; it is classified as a glycoprotein.
• The protein is linked to amyloidosis, forming amyloid fibrils in long-term hemodialysis patients; genetic variants are associated with disease susceptibility.
• Post-translational modifications include glycation and pyrrolidone carboxylic acid formation at the N-terminus.
• Keywords: 3D-structure, Amyloid, Amyloidosis, Direct protein sequencing, Disease variant, Disulfide bond, Glycation, Glycoprotein, Immunity, Immunoglobulin domain, MHC I, Proteomics identification, Pyrrolidone carboxylic acid, Reference proteome, Secreted, Signal.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 21-119, the mature secreted form; verify antibody reactivity under both denaturing and native conditions for WB and FC.
• For WB, the predicted mass is ~13 kDa; use 15% acrylamide gels, optimize transfer time, and include low-molecular-weight markers.
• In IHC-P and IF/ICC, gentle fixation and antigen retrieval are recommended to preserve native cell surface localization.
• For FC, include proper isotype controls; B2M is expressed on most nucleated cells, so confirm specificity in target versus knockout/knockdown models.
• Cross-reactivity is indicated only for human; validate in other species if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with comprehensive IVD raw-material supply—validated antibody pairs, monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing—from concept to clinic. This anti-B2M rabbit monoclonal antibody supports immune presentation and amyloidosis research, and CamelBio's team can assist with assay development, technical consultation, and custom procurement to accelerate your projects.