Anti-BET1L Rabbit Polyclonal Antibody for WB, ELISA - Q9NYM9

Core Product Specifications and Parameters

Biological Background: BET1L Function and Localization

• BET1L, also known as GS15 or GOS-15, is a member of the SNARE protein family that mediates intracellular vesicle trafficking.
• It functions as a vesicle SNARE (v-SNARE) required for the targeting and fusion of retrograde transport vesicles with the Golgi apparatus.
• BET1L is essential for maintaining the structural integrity of the Golgi complex.
• The protein localizes primarily to the Golgi apparatus membrane, including the trans-Golgi network.
• Post-translational modifications include phosphorylation (as indicated by the "Phosphoprotein" keyword).
• It contains a coiled-coil domain and a transmembrane helix, characteristic of SNARE proteins involved in membrane fusion.
• BET1L is implicated in protein transport pathways between the Golgi and endoplasmic reticulum.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-60) of human BET1L, which may be exposed in the native conformation; consider this when designing detection protocols.
• For western blot (WB), the theoretical molecular weight of BET1L is approximately 12.4 kDa; expect a band around 12 kDa under reducing conditions.
• As a polyclonal antibody, batch-to-batch variability can occur; validate the antibody in your specific experimental system, including appropriate positive and negative controls.
• For ELISA, perform a titration experiment to determine the optimal coating or detection concentration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our BET1L antibody supports research in Golgi trafficking and SNARE biology, and we can assist with sourcing rare targets or custom formulations to accelerate your assay development from concept to clinic.