Anti-BET1 Rabbit Polyclonal Antibody for WB and ELISA - O15155

Core Product Specifications and Parameters

Biological Background: BET1 Function and Localization

• BET1 (BET1 homolog), also known as Golgi vesicular membrane-trafficking protein p18, is a SNARE protein encoded by the BET1 gene in humans.
• It is required for ER-to-Golgi vesicular transport, functioning in the docking and fusion of ER-derived vesicles with the cis-Golgi membrane (PubMed:34779586). Related references: PMID:34779586
• BET1 localizes to the endoplasmic reticulum membrane, Golgi apparatus, cis-Golgi network membrane, and Golgi apparatus membrane.
• The protein is expressed in muscle tissue and has been implicated in congenital muscular dystrophy.
• BET1 undergoes alternative splicing and phosphorylation, indicating post-translational regulation.
• Structurally, it contains a coiled-coil domain and a transmembrane helix, typical of SNARE proteins.
• Keywords associated with BET1 include ER-Golgi transport, protein transport, and membrane, underscoring its role in intracellular trafficking.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-94) of human BET1, which includes the cytoplasmic domain; this design may favor detection of the full-length protein.
• For WB, the predicted molecular weight is ~13 kDa; consider using Golgi- or ER-enriched membrane fractions as positive controls, and verify specificity with knockdown or overexpression samples.
• Due to its transmembrane nature, use detergents or denaturing conditions in lysis buffers to ensure efficient solubilization of BET1.
• For ELISA, validate using recombinant BET1 protein or cell lysates; titration of the antibody may be necessary to optimize signal-to-noise ratios.
• Keep in mind that post-translational modifications such as phosphorylation could influence antibody binding or protein migration; adjust detection conditions as needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-BET1 polyclonal antibody supports research into ER-Golgi trafficking, SNARE-mediated membrane fusion, and related disease areas. As your sourcing partner, CamelBio can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and assist with rare target raw-material procurement.