Anti-BEST1 Polyclonal Antibody for WB, ELISA - O76090

Core Product Specifications and Parameters

Biological Background: Bestrophin-1 Function and Localization

• Bestrophin-1 is a calcium-activated chloride channel that mediates anion movement across cell membranes, including chloride and bicarbonate transport (PubMed:11904445, PubMed:12907679, PubMed:18179881). Related references: PMID:11904445, PMID:12907679, PMID:18179881
• It is found in a partially open conformation, resulting in reduced chloride flux (PubMed:35789156). Related references: PMID:35789156
• Beyond chloride, BEST1 transports bicarbonate and neurotransmitters such as glutamate, D-serine, and GABA in a calcium-dependent manner, contributing to synaptic plasticity regulation.
• Localizes predominantly to the basolateral membrane of retinal pigment epithelium cells.
• Encoded by the BEST1 gene, alternative splicing generates multiple isoforms with distinct functional roles.
• Phosphorylation modulates channel activity, and mutations are linked to vitelliform macular dystrophy and retinitis pigmentosa.
• The protein has a molecular weight of approximately 68 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (a.a. 376-585) of human BEST1, so isoforms lacking this domain may not be detected; validate specificity in your target samples.
• Due to the transmembrane nature of BEST1, consider using denaturing conditions and appropriate detergents in Western blotting to ensure complete solubilization.
• For Western blot, an expected band around 68 kDa should be observed; minor bands may appear due to post-translational modifications or processing.
• The antibody cross-reacts with mouse BEST1, enabling its use in mouse retinal pigment epithelium or astrocyte models; however, always confirm cross-reactivity under your experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with a comprehensive range of IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target sourcing solutions. Whether you need BEST1-specific reagents for vision and ion channel research or seek custom development services, CamelBio bridges the gap from concept to clinic with reliable supply and technical support.