Anti-Beclin 1 Polyclonal Antibody for WB, IP, IHC-P, ELISA - Q14457

Core Product Specifications and Parameters

Biological Background: Beclin 1 Function and Localization

• Beclin 1 (BECN1 gene) is a core autophagy protein and a subunit of the class III phosphatidylinositol 3-kinase (PI3K) complexes that generate phosphatidylinositol 3-phosphate, essential for autophagosome initiation and maturation. Related references: PMID:18570871 PMID:21358617 PMID:23184933
• It is required for the formation of specific PI3K complex forms (PI3KC3-C2) and is involved in endocytic trafficking, cytokinesis abscission, and endocytosis. Related references: PMID:20208530 PMID:20643123 PMID:23974797 PMID:25275521
• A 35 kDa Beclin-1-C isoform localizes to mitochondria and promotes apoptosis by inducing BAX translocation and proapoptotic factor release.
• Beclin 1 exhibits a broad subcellular distribution, localizing to the cytoplasm, Golgi, trans-Golgi network, endosomes, endoplasmic reticulum, mitochondria, autophagosomes, and nucleus.
• It is ubiquitously expressed and undergoes diverse post-translational modifications including phosphorylation, acetylation, and ubiquitination, as indicated by keywords such as Acetylation, Phosphoprotein, and Ubl conjugation.
• In microbial infection, Beclin 1 protects against neurovirulent Sindbis virus, underlining its role in antiviral host defense.
• Alternative names include ATG6, VPS30, and coiled-coil myosin-like BCL2-interacting protein.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant protein spanning amino acids 1–280 of human Beclin 1. This N-terminal region is conserved across human, mouse, and rat, aligning with the reported cross-reactivity.
• For Western blot, the predicted molecular weight is approximately 52 kDa; users may also detect a 35 kDa isoform in certain cell types.
• Consider optimizing antigen retrieval for IHC-P and validating the antibody in relevant sample systems.
• This polyclonal antibody may be suitable for immunoprecipitation and ELISA, but individual optimization is recommended.

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