Anti-BCR Polyclonal Antibody for WB, IHC-P, ELISA - P11274

Core Product Specifications and Parameters

Biological Background: BCR Function and Localization

• BCR (Breakpoint cluster region protein) is a multidomain protein that exhibits dual regulatory activities toward small GTPases: a C-terminal GAP domain that stimulates GTP hydrolysis by RAC1, RAC2, and CDC42, and a central DH domain that acts as a GEF for CDC42, RHOA, and RAC1. Related references: PMID:17116687, PMID:1903516, PMID:7479768, PMID:23940119

• The amino-terminal region of BCR contains an intrinsic serine/threonine kinase activity. Related references: PMID:1657398

• BCR functions as a critical negative regulator of neuronal RAC1 activity and modulates macrophage functions, including CSF1-directed motility and phagocytosis, through RAC1 activity modulation. Related references: PMID:17116687

• BCR plays a major role as a RHOA GEF in keratinocytes, involved in focal adhesion formation and keratinocyte differentiation. Related references: PMID:23940119

• Subcellularly, BCR is localized to postsynaptic density, dendritic spines, and axons, particularly concentrated at synapses. This localization supports its role in neuronal signaling and synaptic plasticity.

• Post-translational modifications of BCR include acetylation, phosphorylation, and methylation, which may regulate its enzymatic activities and protein interactions.

• BCR is known as a proto-oncogene; chromosomal rearrangements such as the Philadelphia chromosome create BCR-ABL fusion proteins implicated in leukemias, though the normal BCR protein functions in diverse signaling pathways.

Experimental Guidance and Technical Tips

• Immunogen design: The antibody was raised against a recombinant protein corresponding to amino acids 1-300 of human BCR (NP_004318.3). This region encompasses part of the N-terminal kinase domain, which may allow detection of full-length BCR and potential alternative splice variants that include this sequence.
• Application considerations: This antibody has been validated for Western blotting (WB), immunohistochemistry (paraffin) (IHC-P), and ELISA with human, mouse, and rat samples. For WB, consider the predicted molecular weight of ~143 kDa and use appropriate reducing conditions.
• Cross-reactivity: Since the antibody cross-reacts with mouse and rat BCR, it could be used for comparative studies across these species. However, always validate with appropriate controls in the specific experimental system.
• Technical note: Given BCR's role as a signaling scaffold and enzyme, its expression may be dynamic; optimization of sample preparation and detection conditions may be necessary for reproducible results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to high-quality IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and ancillary reagents. This anti-BCR polyclonal antibody, targeting a key signaling protein with roles in small GTPase regulation, supports oncology and neuroscience research. For bulk orders or custom sourcing requirements, our team offers flexible procurement solutions to accelerate your project from concept to clinic.