Anti-BCMO1 Rabbit Polyclonal Antibody for WB, ELISA - Q9HAY6

Core Product Specifications and Parameters

Biological Background: BCMO1 Function and Localization

• BCMO1 (beta,beta-carotene 15,15'-dioxygenase) is a cytoplasmic enzyme that symmetrically cleaves beta-carotene into two molecules of retinal, a key step in vitamin A production.
• Alternative names include beta-carotene dioxygenase 1 and beta-carotene oxygenase 1, encoded by the BCO1 gene.
• It functions via a dioxygenase mechanism, requiring iron as a metal cofactor for its oxidoreductase activity.
• Subcellularly, BCMO1 is localized to the cytoplasm, predominantly in the cytosol.
• Tissue expression is highest in the retinal pigment epithelium, with additional expression in kidney, testis, liver, brain, small intestine, and colon.
• As a member of the carotenoid cleavage dioxygenase family, BCMO1 plays a critical role in lipid metabolism and provitamin A carotenoid processing.
• UniProt keywords include dioxygenase, iron, metal-binding, lipid metabolism, and reference proteome, highlighting its functional and structural features.

Experimental Guidance and Technical Tips

• For Western blotting, a band corresponding to approximately 63 kDa is expected based on the target protein weight; validate the observed molecular weight in your specific sample system.
• Since the antibody is a polyclonal rabbit pAb, consider lot-to-lot consistency testing if repeatable quantitative results are required.
• In ELISA applications, evaluate the antibody as a capture or detection reagent in a sandwich format, optimizing coating and detection conditions.
• The immunogen spans amino acids 268-547, a region that may be conserved across human, mouse, and rat, consistent with the reported cross-reactivity; verify reactivity in your species of interest if not listed.
• Given its role in retinal formation, this antibody may be useful for studying visual cycle biology or carotenoid metabolism; include appropriate positive controls (e.g., retinal pigment epithelium lysates) and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research institutions with validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-BCMO1 polyclonal antibody serves as a reliable tool for investigating beta-carotene metabolism and retinal production pathways, while CamelBio can further assist in sourcing rare-target raw materials or developing custom assay components to accelerate your diagnostic and research workflows.