Anti-Bcl9 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9D219

Core Product Specifications and Parameters

Biological Background: Bcl9 Function and Localization

• Bcl9 (B-cell CLL/lymphoma 9) is a nuclear protein that functions as a key transcriptional coactivator in the Wnt/β-catenin signaling pathway.
• It was originally identified through frequent rearrangement in B-cell acute lymphoblastic leukemias, highlighting its role in hematological malignancies.
• Bcl9 promotes β-catenin's transcriptional activity by bridging β-catenin to the Pygopus cofactor, facilitating the activation of Wnt target genes.
• The mouse Bcl9 gene encodes a 149 kDa protein with conserved domains essential for its interaction with β-catenin and Pygopus.
• Subcellular localization is predominantly nuclear, consistent with its role in gene transcription.
• Post-translational modifications include acetylation, methylation, and phosphorylation, which may regulate its activity and stability.
• It is expressed in multiple tissues and is crucial for embryonic development and tissue homeostasis, with aberrant activation linked to cancer progression.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1-500 of mouse Bcl9, encompassing the N-terminal region. This design may help detect full-length and possibly truncated isoforms.
• For Western blotting (WB), the predicted molecular weight is 149 kDa. A clear band near 150 kDa should be expected; use appropriate high-molecular-weight markers and transfer conditions.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), nuclear staining patterns are anticipated; co-staining with a nuclear marker may aid signal localization.
• Pre-incubation with the immunogen peptide can serve as a blocking control to verify band specificity in WB.
• Cross-reactivity with human and rat suggests conserved epitopes; consider validating in the relevant sample system for each species.
• Follow standard ELISA protocols with titration of antibody and antigen; sensitivity may be impacted by buffer conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. As a trusted sourcing partner, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw materials to accelerate your Wnt signaling and cancer biomarker projects. Our inventory includes this anti-Bcl9 antibody, ready for integration into your assay development workflows.