Anti-Bcl9 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9D219

Core Product Specifications and Parameters

Biological Background: Bcl9 Function and Localization

• Bcl9 (B-cell CLL/lymphoma 9 protein) is a nuclear coactivator that enhances beta-catenin-mediated transcription, playing a key role in the Wnt signaling pathway.
• The protein is localized to the nucleus, consistent with its transcriptional regulatory function.
• Bcl9 is subject to post-translational modifications including acetylation, methylation, and phosphorylation, which may modulate its activity.
• The gene is conserved across human, mouse, and rat, with the mouse protein (UniProt Q9D219) having a calculated molecular weight of ~149 kDa.
• Alias names include Gm130 and other identifiers, reflecting its discovery in different contexts.
• Bcl9 is implicated in cancer development, particularly through its role in Wnt-driven transcriptional programs.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a sequence within amino acids 1278-1377 of mouse Bcl9 (NP_084209.3), suggesting epitope recognition in the C-terminal region. Validate specificity using Wnt-activated and Wnt-inhibited cell models.
• For WB, expect a band at ~149 kDa; use appropriate gel percentage (e.g., 6-8% SDS-PAGE) and optimized transfer conditions for high molecular weight proteins.
• IHC-P may require antigen retrieval; consider citrate buffer (pH 6.0) or EDTA (pH 9.0) with heat induction, and titrate the antibody concentration for each tissue type.
• ELISA can be performed with recombinant Bcl9 fragments or validated lysates; include appropriate controls to confirm binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a one-stop platform for IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-Bcl9 antibody supports Wnt signaling research and the development of cancer diagnostics, with reliable cross-reactivity across human, mouse, and rat samples. For rare targets or custom sourcing needs, CamelBio’s technical consulting can help streamline your procurement workflow.