Anti-Bcl9 Rabbit Polyclonal Antibody for WB and ELISA - O00512

Core Product Specifications and Parameters

Biological Background: Bcl9 Function and Localization

• Bcl9 (B-cell CLL/lymphoma 9 protein), also known as Protein legless homolog, is a key component of the Wnt signaling pathway. It promotes β-catenin's transcriptional activity, facilitating target gene activation in response to Wnt ligands (By similarity).
• The protein localizes predominantly to the nucleus, consistent with its role in transcriptional co-activation.
• Bcl9 is subject to various post-translational modifications, including phosphorylation, acetylation, and methylation, which may modulate its activity and stability.
• As a proto-oncogene, Bcl9 is implicated in tumorigenesis; chromosomal rearrangements involving BCL9 have been identified in certain B-cell malignancies.
• Tissue expression analysis reveals Bcl9 at low levels in thymus, prostate, testis, ovary, and small intestine, with even lower expression in spleen, colon, and blood.

Experimental Guidance and Technical Tips

• The antibody was raised against an N-terminal fragment (aa 1-100), which is expected to recognize full-length Bcl9.
• For Western blot, a protein weight of approximately 149 kDa is predicted; consider using a high-molecular-weight marker and appropriate gel percentage for transfer.
• For ELISA, validate coating conditions and detection system in your specific assay setup to ensure optimal performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted IVD raw-material partner, offering this anti-Bcl9 polyclonal antibody alongside a broad portfolio of validated antibodies, bulk ancillary reagents, and rare target sourcing capabilities. Whether you need assay development support or scalable supply solutions, our one-stop service bridges research and diagnostic manufacturing. Let CamelBio simplify your procurement from concept to clinic.