Anti-BCL6 Rabbit Monoclonal Antibody for Flow Cytometry and ELISA - P41182

Core Product Specifications and Parameters

Biological Background: BCL6 Function and Localization

• BCL6 (B-cell lymphoma 6 protein) is a zinc finger and BTB domain-containing transcriptional repressor encoded by the BCL6 gene; also known as BCL5, LAZ3, ZBTB27, and ZNF51.
• It functions as a master regulator of germinal center (GC) formation and antibody affinity maturation, repressing genes involved in differentiation, inflammation, apoptosis, and cell cycle control.
• BCL6 binds directly to the DNA sequence 5'-TTCCTAGAA-3' or indirectly represses transcription factors, recruiting corepressors and histone deacetylases to target gene promoters.
• The protein is localized exclusively in the nucleus, consistent with its role as a DNA-binding transcription factor.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitin conjugation, which modulate its stability and activity.
• BCL6 is predominantly expressed in germinal center B-cells and T-cells, as well as primary immature dendritic cells, highlighting its role in adaptive immunity.
• It is classified as a proto-oncogene, with chromosomal rearrangements (e.g., translocations) and dysregulation implicated in B-cell lymphomas.
• BCL6 also plays roles in controlling neurogenesis and macrophage proliferation, indicating broader biological functions beyond lymphocyte biology.

Experimental Guidance and Technical Tips

• Given the immunogen design against the mid-region of human BCL6, this antibody may recognize both full-length and certain isoforms; verify in your sample system.
• For intracellular flow cytometry, ensure adequate cell fixation and permeabilization to allow antibody access to the nuclear-localized BCL6 protein.
• For ELISA applications, optimize coating antigen and antibody concentration to achieve optimal signal-to-noise ratio; consider using recombinant BCL6 protein as standard.
• Since BCL6 is a transcription factor with low-abundance expression, signal amplification strategies may be necessary in some detection systems.
• Cross-reactivity is confirmed for human; for other species, perform sequence alignment of the immunogen region to predict potential reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated sourcing partner for IVD raw materials, offering a comprehensive portfolio that includes validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. For BCL6-related research and diagnostic assay development, we provide high-quality monoclonal antibodies such as this BCL6 clone to accelerate your biomarker studies and immunoassay development. From concept to clinic, CamelBio streamlines your supply chain with technical expertise and tailored sourcing solutions.