Anti-BCL6 Monoclonal Antibody () for Intracellular Flow Cytometry - P41182

Core Product Specifications and Parameters

Biological Background: BCL6 Function and Localization

• BCL6 (B-cell lymphoma 6) is a transcriptional repressor critical for germinal center formation and antibody affinity maturation, encoded by the BCL6 gene (GeneID: 604) with multiple isoforms generated by alternative splicing.
• It binds the DNA consensus sequence 5'-TTCCTAGAA-3' and recruits corepressor complexes, including histone deacetylases, to silence target genes involved in differentiation, apoptosis, and cell cycle control in GC B-cells.
• BCL6 autoregulates its own expression and indirectly upregulates AICDA by repressing microRNAs such as miR-155, thereby promoting immunoglobulin class switch recombination and somatic hypermutation while suppressing p53-dependent apoptosis.
• In follicular helper T-cells (TFH), BCL6 drives the TFH gene program and inhibits differentiation into TH1, TH2, and TH17 lineages, and is essential for immunological memory in both B- and T-cells.
• It also represses macrophage proliferation by competing with STAT5 for binding motifs on target genes like CCL2 and CCND2, and mediates cell cycle arrest in GC B-cells under genotoxic stress via p53-dependent and independent pathways.
• Beyond immunity, BCL6 controls neurogenesis by altering NOTCH-dependent transcription at targets like HES5, recruiting the deacetylase SIRT1 to induce epigenetic silencing and neuronal differentiation.
• Subcellularly, BCL6 localizes exclusively to the nucleus, consistent with its role as a DNA-binding transcription factor.
• Post-translational modifications include phosphorylation and acetylation, and the protein features an N-terminal BTB/POZ domain and C-terminal zinc fingers, classifying it as a zinc-finger transcriptional repressor.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 235–350 (central region outside the BTB domain), which may yield an antibody recognizing both major isoforms (72 kDa and 78 kDa); validate specificity in your target cell type.
• As this antibody is directly conjugated to ABflo® 488, it is suitable for intracellular flow cytometry; optimize fixation and permeabilization steps for your nuclear target to ensure adequate epitope accessibility.
• Since BCL6 is expressed transiently during GCB differentiation, consider using appropriate gating strategies and co-staining with markers (e.g., CD38, CD20) to identify GC B-cells or TFH cells in human samples.
• Confirm cross‑reactivity with your samples, as documented reactivity is limited to human; perform additional validation if working with non‑human primate or other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw materials, offering a wide portfolio of validated antibody pairs, recombinant proteins, and ancillary reagents to support immunoassay development from concept to clinic. For researchers and manufacturers focused on lymphoid malignancies or immunophenotyping, we can source rare‑target materials like anti‑BCL6 antibodies and provide custom conjugation options to streamline your assay workflows. Our integrated supply chain and technical consulting simplify procurement, ensuring you receive high‑quality, lot‑to‑lot consistent reagents for diagnostic and research applications.