Anti-BCL3 Polyclonal Antibody for WB, IF/ICC, ELISA - P20749

Core Product Specifications and Parameters

Biological Background: BCL3 Function and Localization

• BCL3 (B-cell lymphoma 3 protein, also known as BCL4 or D19S37) is a proto-oncogene and member of the IκB family, characterized by ankyrin repeat domains that mediate protein-protein interactions.
• It functions as a critical regulator of NF-κB signaling: in the cytoplasm, BCL3 inhibits nuclear translocation of the p50 subunit, while in the nucleus, it acts as a transcriptional coactivator to promote NF-κB target gene expression.
• Through this dual regulatory mechanism, BCL3 contributes to the control of cell proliferation, survival, and immune responses, linking it to oncogenic processes.
• Subcellular localization is dynamic; BCL3 can be found in the cytoplasm (often perinuclear) and the nucleus, shuttling between compartments to modulate gene transcription.
• The protein undergoes post-translational modifications including phosphorylation and ubiquitination (Ubl conjugation), which may impact its stability, localization, and activity.
• Chromosomal rearrangements involving the BCL3 locus are associated with certain B-cell leukemias/lymphomas, underscoring its role in hematologic malignancies.
• Keywords for P20749 include: Activator, ANK repeat, Chromosomal rearrangement, Cytoplasm, Nucleus, Phosphoprotein, Proto-oncogene, Repressor, Transcription regulation, Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (aa 300-400) of human BCL3, which may recognize full-length endogenous protein as well as isoforms containing this region. Verify cross-reactivity with mouse/rat BCL3 in the target sample system.
• For Western blotting, a band around 48 kDa is expected. Use appropriate positive controls (e.g., HEK293, HeLa lysates) and consider nuclear/cytoplasmic fractionation to enrich signal.
• In immunofluorescence/ICC, include controls for nuclear vs. cytoplasmic staining, as BCL3 localization can vary. Use permeabilization parameters optimized for nuclear proteins.
• For ELISA, the antibody may be used for capture or detection; perform pilot titration experiments to determine optimal working concentration.
• Since BCL3 expression can be context-dependent, consider stimulation with NF-κB activators (e.g., TNF-α, PMA/ionomycin) to upregulate protein levels in certain cell types.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines procurement for diagnostic manufacturers and research labs by providing a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For BCL3-related research and assay development, we support the supply of high-quality antibodies and related recombinant proteins. Our one-stop approach bridges the gap from discovery to clinical application, helping you accelerate development with reliable, traceable raw materials.