Anti-Bcl10 Monoclonal Antibody for WB, ELISA - O95999

Core Product Specifications and Parameters

Biological Background: Bcl10 Function and Localization

• Bcl10 (B-cell lymphoma/leukemia 10) is a key signaling adapter in both innate and adaptive immunity, bridging CARD domain-containing proteins (CARD9, CARD11, CARD14) to NF-kappa-B and MAP kinase activation. Related references: PMID:10187770, PMID:10364242, PMID:10400625
• It acts downstream of activated CARD proteins, which form nucleating helical templates that recruit BCL10 via CARD-CARD interactions, leading to BCL10 polymerization and formation of the CBM complex with MALT1. Related references: PMID:24074955
• The CBM complex triggers NF-kappa-B and p38 MAP kinase pathways, driving expression of pro-inflammatory cytokines and chemokines. Related references: PMID:18287044, PMID:24074955, PMID:27777308
• BCL10 is activated by CARD9 downstream of C-type lectin receptors, essential for antifungal immunity, and by CARD11 downstream of T-cell and B-cell receptors. Related references: PMID:26488816, PMID:18264101, PMID:18287044, PMID:24074955
• It also promotes apoptosis and pro-caspase-9 maturation, acting through NIK and IKK. Related references: PMID:10187815
• Subcellularly, BCL10 localizes to the cytoplasm, perinuclear region, and membrane rafts.
• It is ubiquitously expressed in human tissues.
• BCL10 is associated with chromosomal rearrangements in MALT lymphomas, contributing to constitutive NF-kappa-B activation (Keywords: Chromosomal rearrangement, Disease variant).

Experimental Guidance and Technical Tips

• The immunogen spans full-length human BCL10 (amino acids 1–233), enabling detection of total BCL10 in Western blotting.
• For ELISA applications, consider pairing with a detection antibody that recognizes a non-overlapping epitope to avoid competition.
• Validate the antibody in the relevant human sample system; cross-reactivity with other species has not been reported.
• Due to BCL10's role in immune signaling, activation-induced changes (e.g., phosphorylation, complex formation) may require optimization of lysis and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research teams with reliable access to validated IVD raw materials. For studies on immune signaling, NF-kappa-B pathways, and CBM complex formation, this Bcl10 antibody is part of a broader portfolio that includes optimized monoclonal and polyclonal antibodies, validated antibody pairs, and bulk ancillary reagents. Our one-stop sourcing model also assists with rare-target raw-material identification, helping you accelerate development from concept to clinic.