Anti-Bcl10 (KO Validated) Rabbit Polyclonal Antibody for WB, ELISA - O95999

Core Product Specifications and Parameters

Biological Background: Bcl10 Function and Localization

• BCL10 is a scaffold protein linking CARD domain-containing receptors to NF-κB and p38 MAPK pathways, critical for immune cell activation and cytokine production. Related references: PMID:10187770 PMID:10364242 PMID:10400625 PMID:24074955
• It forms the CBM complex (CARD-BCL10-MALT1) upon CARD protein oligomerization, nucleating filamentous assemblies that propagate immune signals. Related references: PMID:24074955
• Activated by CARD11 downstream of T-cell receptor (TCR) and B-cell receptor (BCR) for adaptive immunity. Related references: PMID:18264101 PMID:18287044 PMID:24074955
• Activated by CARD9 downstream of C-type lectin receptors, essential for antifungal defense. Related references: PMID:26488816
• BCL10 also promotes apoptosis and caspase-9 maturation, contributing to its tumor suppressor role. Related references: PMID:10187815
• Subcellular localization includes cytoplasm, perinuclear region, and membrane rafts, consistent with its role in assembling signaling complexes at membranes.
• Ubiquitously expressed, with functions in various tissues and immune cells.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-233) of human Bcl10, containing the CARD domain; this region is highly conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For WB analysis, the predicted molecular weight is 26 kDa; consider using a 10-12% SDS-PAGE gel and validate with appropriate loading controls. KO validation confirms specificity.
• For ELISA, optimize coating conditions and validate with recombinant Bcl10 or cell lysates.
• As with any polyclonal antibody, lot-to-lot variability may occur; consider including positive and negative controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our supply capabilities include validated antibody pairs, optimized monoclonal/polyclonal antibodies like this anti-Bcl10 antibody for NF-κB pathway research, bulk ancillary reagents, and rare target raw-material sourcing. Partner with CamelBio to streamline your assay development and secure a reliable material supply.