Anti-Bcl-XL Rabbit Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - Q07817

Core Product Specifications and Parameters

Biological Background: Bcl-XL Function and Localization

• Bcl-2-like protein 1 (Bcl-X) is an apoptosis regulator encoded by the BCL2L1 gene, producing two major isoforms via alternative splicing: Bcl-X(L) (long) and Bcl-X(S) (short).
• Bcl-X(L) potently inhibits cell death by blocking the voltage-dependent anion channel (VDAC) on the mitochondrial membrane, preventing release of cytochrome c (CYC1) and subsequent caspase activation. It also regulates the G2 checkpoint and progression to cytokinesis during mitosis.
• Isoform Bcl-X(S) promotes apoptosis, contrasting with the anti-apoptotic role of Bcl-X(L).
• Bcl-X(L) localizes to mitochondrial inner and outer membranes, mitochondrial matrix, and also to synaptic vesicle membranes, cytoplasm, centrosomes, and nuclear membranes, reflecting its multifaceted roles.
• In neurons, Bcl-X(L) regulates presynaptic plasticity by controlling neurotransmitter release and recovery, axonal mitochondrial number, and synaptic vesicle cluster size. During synaptic stimulation, it increases mitochondrial ATP availability via F(1)F(0) ATP synthase regulation and facilitates endocytic vesicle retrieval through DMN1L GTPase activation (PubMed:17418785). Related references: PMID:17418785
• Bcl-X(L) may attenuate inflammation by impairing NLRP1-inflammasome activation, thereby reducing CASP1 activation and IL1B release.
• Tissue-specific expression: Bcl-X(S) is highly expressed in cells with high turnover (e.g., developing lymphocytes), while Bcl-X(L) predominates in long-lived postmitotic cells like adult brain.
• Post-translational modifications include phosphorylation and ubiquitin conjugation, as indicated by keywords.

Experimental Guidance and Technical Tips

• Immunogen corresponds to the N-terminal region (aa 1-80) of human Bcl-XL, conserved across human, mouse, and rat, supporting broad reactivity.
• For Western blotting, expect a band around 26–30 kDa for Bcl-X(L). Bcl-X(S) may appear as a smaller band (~20 kDa) depending on tissue/cell type. Use appropriate positive controls such as HeLa, Jurkat, or brain lysates.
• For IHC-P, validate with known Bcl-XL positive tissues (e.g., brain, lymphoid tissues); antigen retrieval may be necessary.
• For IF/ICC, Bcl-XL localizes to mitochondria; co-staining with mitochondrial markers (e.g., MitoTracker) is recommended.
• For ELISA, the antibody can be used as a capture antibody in sandwich ELISA when paired with a matched detection antibody; optimize coating conditions.
• As a polyclonal antibody, verify lot-to-lot consistency in your intended assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your one-stop sourcing partner for IVD raw materials, connecting diagnostic manufacturers and research laboratories with high-quality reagents like the Bcl-XL Rabbit pAb for apoptosis and mitochondrial studies. We offer validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and can source rare-target raw materials to accelerate your assay development from concept to clinic.