Anti-Bcl-XL Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q07817

Core Product Specifications and Parameters

Biological Background: Bcl-2-like protein 1 (Bcl-X) Function and Localization

• Bcl-2-like protein 1 (also known as Bcl-X) is encoded by the BCL2L1 gene; alternative splicing produces two major isoforms: Bcl-X(L) (anti-apoptotic) and Bcl-X(S) (pro-apoptotic).
• Acts as a potent inhibitor of apoptosis by blocking VDAC to prevent cytochrome c release, and regulates the G2 mitotic checkpoint.
• Isoform Bcl-X(L) modulates presynaptic plasticity, mitochondrial ATP production, and synaptic vesicle endocytosis; also attenuates inflammation by inhibiting NLRP1-inflammasome activation, reducing CASP1 and IL1B release (PubMed:17418785). Related references: PMID:17418785
• Isoform Bcl-X(S) promotes apoptosis, contrasting with the protective role of Bcl-X(L).
• Localizes to the mitochondrial inner and outer membranes, cytoplasm, synaptic vesicles, centrosome, and nuclear membrane.
• Tissue specificity: Bcl-X(L) is predominantly expressed in long-lived postmitotic cells like adult brain neurons, while Bcl-X(S) is found in cells with high turnover such as developing lymphocytes.
• Keywords: apoptosis, alternative splicing, mitochondrial membrane, transmembrane, synapse, phosphoprotein.

Experimental Guidance and Technical Tips

• For Western blotting, expect a band near 26 kDa; positive control lysates from cells known to express Bcl-XL (e.g., HeLa, Jurkat) are recommended.
• The immunogen targets the N-terminal region (aa 1-80), common to both Bcl-X(L) and Bcl-X(S) isoforms; validation for isoform specificity should be performed in the relevant experimental system.
• In IHC-P, optimize antigen retrieval and validate staining in tissues with known expression patterns (e.g., brain for Bcl-X(L) or lymphoid organs for Bcl-X(S)).
• For ELISA, the antibody may be used for detection of recombinant or native Bcl-XL; include appropriate controls and titrate the antibody to determine optimal working concentration.
• Cross-reactivity with human, mouse, and rat Bcl-XL has been reported; verify reactivity in your sample species if it differs from the tested ones.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, labs, and research institutes with a comprehensive portfolio of IVD raw materials, including this high-quality anti-Bcl-XL monoclonal antibody for apoptosis and mitochondrial research. Beyond this antibody, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing support for rare target raw materials. Whether you are developing an IVD test or scaling up research reagents, CamelBio provides one-stop access to streamline your workflow from concept to clinic.