Anti-Bcl-W Polyclonal Antibody for WB, ELISA - Q92843

Core Product Specifications and Parameters

Biological Background: Bcl-W Function and Localization

• Bcl-W (BCL2L2) is a member of the Bcl-2 protein family, functioning as an apoptosis regulator that promotes cell survival by blocking dexamethasone-induced apoptosis.
• It localizes to the mitochondrial membrane, where it mediates the survival of postmitotic Sertoli cells by suppressing the death-promoting activity of BAX.
• The protein undergoes alternative splicing, producing multiple isoforms, and is subject to post-translational modifications including acetylation, methylation, and phosphorylation.
• Bcl-W is widely expressed at the protein level, with highest levels in brain, spinal cord, testis, pancreas, heart, spleen, and mammary glands; moderate levels in thymus, ovary, and small intestine; and undetectable in salivary gland, muscle, or liver.
• It is also expressed in cell lines of myeloid, fibroblast, and epithelial origin but absent in most lymphoid cell lines, indicating a tissue- and cell-type-specific regulation.
• Keywords associated with Bcl-W include 3D-structure, alternative splicing, apoptosis, membrane, methylation, mitochondrion, and phosphoprotein, reflecting its multifaceted roles in cellular homeostasis.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering the N-terminal region (amino acids 1–193) of human Bcl-W; therefore, the antibody may recognize multiple isoforms if they share this epitope.
• For Western blotting, consider using positive controls from tissues or cell lines with high endogenous Bcl-W expression, such as brain or testis lysates.
• ELISA applications may require optimization of coating antigen and antibody dilution; pairing with a validated detection antibody could improve signal specificity.
• Cross-reactivity with mouse and rat is indicated; however, verify performance in your specific model system as sequence homology may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, providing diagnostic manufacturers and research laboratories with reliable access to validated antibodies like this anti-Bcl-W polyclonal antibody for apoptosis research. We offer one-stop supply solutions including optimized antibody pairs, bulk monoclonal/polyclonal antibodies, ancillary reagents, and custom sourcing for rare targets, supporting your projects from development to clinical application.