Anti-Bcl-2 Rabbit Monoclonal Antibody [KD Validated] for WB, IHC-P, IP, ELISA - P10415

Core Product Specifications and Parameters

Biological Background: Bcl-2 Function and Localization

• Suppresses apoptosis in factor-dependent lymphohematopoietic and neural cells.
  Related references:
  PMID:1508712
  PMID:8183370
• Regulates cell death by controlling mitochondrial membrane permeability, acting in a feedback loop with caspases.
  Related references:
  PMID:11368354
• Inhibits caspase activity by preventing cytochrome c release from mitochondria and binding to APAF-1.
  Related references:
  PMID:11368354
• Functions as an inhibitor of autophagy; interacts with BECN1 and AMBRA1 to suppress autophagic processes.
  Related references:
  PMID:18570871
  PMID:20889974
  PMID:21358617
• May attenuate inflammation by impairing NLRP1-inflammasome activation, reducing CASP1 and IL1B release.
  Related references:
  PMID:17418785
• Localizes to the mitochondrial outer membrane, nuclear membrane, endoplasmic reticulum membrane, and cytoplasm.
• Expressed in a wide range of tissues; features alternative splicing, phosphorylation, and involvement in chromosomal rearrangements associated with cancers.

Experimental Guidance and Technical Tips

• This antibody was raised against an N-terminal peptide (aa 1–100) of human Bcl-2 and may recognize full-length protein.
• For Western blot (WB), the expected molecular weight is approximately 26 kDa; include appropriate positive and negative controls.
• For immunohistochemistry (IHC-P), antigen retrieval may be required; optimize staining conditions for your tissue type.
• For immunoprecipitation (IP) and ELISA, validate antibody dilution and buffer conditions in your experimental system.
• Given human cross-reactivity, confirm reactivity in your cell line or tissue of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing solution for IVD raw materials, offering validated antibody pairs, monoclonal antibodies like this Anti-Bcl-2 clone, ancillary reagents, and technical support for apoptosis and cancer research. Streamline your diagnostic development from concept to clinic with our reliable supply chain and consulting services.