Anti-Bcl-2 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P49950

Core Product Specifications and Parameters

Biological Background: Bcl-2 Function and Localization

• Apoptosis regulator Bcl-2 suppresses apoptosis in a variety of cell systems, including factor-dependent lymphohematopoietic and neural cells.
• It regulates cell death by controlling mitochondrial membrane permeability and functions in a feedback loop with caspases, inhibiting caspase activity by preventing cytochrome c release and/or binding APAF-1.
• Bcl-2 also acts as an inhibitor of autophagy under non-starvation conditions by interacting with BECN1 and AMBRA1.
• It may attenuate inflammation by impairing NLRP1-inflammasome activation, thereby reducing CASP1 activation and IL1B release.
• Subcellularly, Bcl-2 localizes to the mitochondrial outer membrane, nucleus membrane, endoplasmic reticulum membrane, and cytoplasm.
• Expressed in many tissues, with highest levels in reproductive tissues. In brain, it is found in specific neuronal populations like olfactory bulb mitral cells, hippocampal granule/pyramidal neurons, pontine nuclei, and cerebellar granule neurons, with higher prenatal expression in neuroepithelium and cortical plate.
• Keywords: Apoptosis, Autophagy, Endoplasmic reticulum, Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus, Phosphoprotein, Reference proteome, Transmembrane, Transmembrane helix, Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminus (aa 1-100) of rat Bcl-2, which may contribute to cross-reactivity (confirmed for mouse and rat).
• Suitable for Western blotting (WB), immunohistochemistry on paraffin sections (IHC-P), and ELISA. For IHC-P, consider antigen retrieval protocols appropriate for nuclear/membrane targets.
• Always validate antibody performance in your specific sample system and include appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing platform for IVD raw materials, supporting diagnostic manufacturers and research labs from concept to clinic. This Bcl-2 Rabbit mAb is part of our portfolio for apoptosis and cancer research, and we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing. Contact us to streamline your procurement and accelerate your diagnostic development.