Anti-Bcl-2 (PPP1R50) Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P10415

Core Product Specifications and Parameters

Biological Background: Bcl-2 Function and Localization

• Apoptosis regulator that suppresses cell death in a variety of cell systems, including factor-dependent lymphohematopoietic and neural cells. Related references: PMID:1508712, PMID:8183370
• Controls mitochondrial membrane permeability and functions in a feedback loop with caspases to inhibit apoptosis by preventing cytochrome c release and binding APAF-1. Related references: PMID:11368354
• Inhibits autophagy by interacting with BECN1 and AMBRA1 under non-starvation conditions. Related references: PMID:18570871, PMID:20889974, PMID:21358617
• May attenuate inflammation by impairing NLRP1-inflammasome activation, reducing CASP1 activation and IL1B release. Related references: PMID:17418785
• Localizes to the mitochondrial outer membrane, nucleus membrane, endoplasmic reticulum membrane, and cytoplasm.
• Encoded by the BCL2 gene, a proto-oncogene subject to chromosomal rearrangement and alternative splicing, associated with various disease variants.
• Post-translational modifications include phosphorylation and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-100 of human Bcl-2, suggesting the antibody may recognize the N-terminal region; consider peptide blocking to confirm specificity in your assay.
• For WB, a band around 26 kDa is expected; validate the molecular weight in your sample system and use appropriate positive controls (e.g., Jurkat, MCF-7 cell lysates).
• In IHC-P and IF/ICC, signal is anticipated at mitochondria, ER, and nuclear membranes; optimize antigen retrieval and permeabilization methods.
• This antibody has been reported for IP and ELISA; however, individual experimental conditions (buffer, incubation times) should be optimized.
• Cross-reactivity with mouse and rat samples has been noted; verify reactivity with your target species if different.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories with a wide range of validated antibodies, recombinant proteins, and ancillary reagents. This Bcl-2 polyclonal antibody is ideal for apoptosis and oncology research, enabling reliable performance in applications such as WB, IHC, and ELISA. For bulk requirements or custom sourcing of rare targets, our team is ready to assist.