Anti-Bcl-2 Polyclonal Antibody for WB - P10415

Core Product Specifications and Parameters

Biological Background: Bcl-2 Function and Localization

• Suppresses apoptosis in factor-dependent lymphohematopoietic and neural cells (PubMed:1508712, PubMed:8183370). Related references: PMID:1508712 | PMID:8183370
• Controls mitochondrial membrane permeability and inhibits caspase activity by preventing cytochrome c release or binding to APAF-1 (PubMed:11368354). Related references: PMID:11368354
• Acts as an inhibitor of autophagy by interacting with BECN1 and AMBRA1 during non-starvation conditions (PubMed:18570871, PubMed:20889974, PubMed:21358617). Related references: PMID:18570871 | PMID:20889974 | PMID:21358617
• May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785). Related references: PMID:17418785
• Localized to the mitochondrial outer membrane, nucleus membrane, endoplasmic reticulum membrane, and cytoplasm.
• Widely expressed in a variety of tissues.
• Undergoes alternative splicing and is subject to post-translational modifications including phosphorylation and ubiquitin-like conjugation.
• Identified as a proto-oncogene; chromosomal rearrangements involving BCL2 are associated with certain lymphomas.

Experimental Guidance and Technical Tips

• The immunogen is a full-length recombinant human Bcl-2 protein (aa 1–239); consider possible recognition of splice variants sharing this sequence.
• For Western blotting, expect a band at approximately 26 kDa; optimize primary antibody dilution for your specific lysate and detection system.
• In immunohistochemistry, validate with appropriate positive and negative tissue controls.
• For immunofluorescence, permeabilization conditions may influence staining; test with your cellular model.
• ELISA performance may require optimization of coating antigen and detection conditions.

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